Repertoire of Escherichia coli agonists sensed by innate immunity receptors of the bovine udder and mammary epithelial cells.
Repertoire of Escherichia coli agonists sensed by innate immunity receptors of the bovine udder and mammary epithelial cells.
复制标题
DOI：
10.1186/1297-9716-43-14
复制
发表时间：
2012-02-13
影响因子：
4.4
通讯作者：
Germon P
中科院分区：
文献类型：
--
作者：
Porcherie A;Cunha P;Trotereau A;Roussel P;Gilbert FB;Rainard P;Germon P
大肠杆菌是奶牛临床乳腺炎的常见原因。研究表明，大肠杆菌进入乳腺管腔后，需要乳腺做出及时反应来控制感染，这意味着细菌的早期检测至关重要。然而，除了脂多糖（LPS）之外，人们对乳腺先天免疫系统检测到的细菌成分知之甚少。我们通过使用已识别模式识别受体（PRR）的细菌激动剂的纯化或合成分子替代物，研究了大肠杆菌乳腺炎期间乳房和牛乳腺上皮细胞（bMEC）感知的潜在细菌激动剂的全部成分。 CXCL8 的产生和乳汁中白细胞的流入分别是刺激培养的 bMEC 和攻击乳房的反应性读数。定量 PCR 显示培养物中的 bMEC 表达核苷酸寡聚化结构域受体 NOD1 和 NOD2，以及 Toll 样受体 TLR1、TLR2、TLR4 和 TLR6，但几乎不表达 TLR5。与表达数据一致，bMEC 被证明对同源激动剂 C12-iE-DAP (NOD1)、Pam3CSK4 (TLR1/2)、Pam2CSK4 (TLR2/6)、纯 LPS (TLR4) 发生反应，但不与鞭毛蛋白 (TLR5) 发生反应。由于乳房对 NOD1 和 TLR5 激动剂的反应性从未有过报道，我们测试了乳腺是否对乳房内输注 C12-iE-DAP 或鞭毛蛋白有反应。乳房对 C12-iE-DAP 有反应，但对鞭毛蛋白没有反应，这与 bMEC 的反应性一致。这些结果扩展了我们对牛乳腺对先天免疫系统细菌激动剂的反应性的了解，并表明大肠杆菌可以被包括 NOD1 在内的几种 PRR 识别，但出乎意料的是不能被 TLR5 识别。乳腺感知大肠杆菌的方式可能会影响先天免疫反应，并最终导致大肠杆菌乳腺炎的结果。
Escherichia coli is a frequent cause of clinical mastitis in dairy cows. It has been shown that a prompt response of the mammary gland after E. coli entry into the lumen of the gland is required to control the infection, which means that the early detection of bacteria is of prime importance. Yet, apart from lipopolysaccharide (LPS), little is known of the bacterial components which are detected by the mammary innate immune system. We investigated the repertoire of potential bacterial agonists sensed by the udder and bovine mammary epithelial cells (bMEC) during E. coli mastitis by using purified or synthetic molecular surrogates of bacterial agonists of identified pattern-recognition receptors (PRRs). The production of CXCL8 and the influx of leucocytes in milk were the readouts of reactivity of stimulated cultured bMEC and challenged udders, respectively. Quantitative PCR revealed that bMEC in culture expressed the nucleotide oligomerization domain receptors NOD1 and NOD2, along with the Toll-like receptors TLR1, TLR2, TLR4, and TLR6, but hardly TLR5. In line with expression data, bMEC proved to react to the cognate agonists C12-iE-DAP (NOD1), Pam3CSK4 (TLR1/2), Pam2CSK4 (TLR2/6), pure LPS (TLR4), but not to flagellin (TLR5). As the udder reactivity to NOD1 and TLR5 agonists has never been reported, we tested whether the mammary gland reacted to intramammary infusion of C12-iE-DAP or flagellin. The udder reacted to C12-iE-DAP, but not to flagellin, in line with the reactivity of bMEC. These results extend our knowledge of the reactivity of the bovine mammary gland to bacterial agonists of the innate immune system, and suggest that E. coli can be recognized by several PRRs including NOD1, but unexpectedly not by TLR5. The way the mammary gland senses E. coli is likely to shape the innate immune response and finally the outcome of E. coli mastitis.
登录
查看更多内容
影响因子：
64.8
作者：
Bouskra, Djahida;Brezillon, Christophe;Eberl, Gerard
通讯作者：
Eberl, Gerard
影响因子：
5.4
作者：
Fritz, JH;Girardin, SE;Adib-Conquy, M
通讯作者：
Adib-Conquy, M
DOI：
10.1128/cdli.11.3.463-472.2004
发表时间：
2004-05-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Bannerman, DD;Paape, MJ;Rainard, P
通讯作者：
Rainard, P
影响因子：
6.7
作者：
Lysenko ES;Clarke TB;Shchepetov M;Ratner AJ;Roper DI;Dowson CG;Weiser JN
通讯作者：
Weiser JN
影响因子：
3.1
作者：
Mosa, Ahmed;Trumstedt, Christian;Rottenberg, Martin E.
通讯作者：
Rottenberg, Martin E.