The entomopathogenic fungus Metarhizium robertsii communicates with the insect host Galleria mellonella during infection.
The entomopathogenic fungus Metarhizium robertsii communicates with the insect host Galleria mellonella during infection.
复制标题
DOI：
10.1080/21505594.2017.1405190
复制
发表时间：
2018-01-01
期刊：
影响因子：
5.2
通讯作者：
Vilcinskas A
中科院分区：
文献类型：
--
作者：
Mukherjee K;Vilcinskas A
寄生真菌是唯一能够通过昆虫的蛋白质外骨骼直接感染昆虫宿主的病原体。表皮的渗透需要真菌酶的释放，包括蛋白酶，其作为毒力因子。昆虫可以感知真菌感染并激活先天免疫反应，包括合成抗真菌肽和蛋白酶抑制剂，以中和传入的蛋白酶。这种研究充分的宿主反应是由组蛋白乙酰化/去乙酰化的表观遗传调控。在这里，我们表明，昆虫病原真菌可以反过来感觉到存在的昆虫衍生的抗真菌肽和蛋白酶抑制剂，并通过诱导胰凝乳蛋白酶样蛋白酶和金属蛋白酶，降解宿主衍生的防御分子的合成。这种反应的速度取决于真菌菌株的毒力。我们通过LC/MS和RT-PCR分析证实了病原体对宿主来源的防御分子的反应的特异性，并将此过程与组蛋白乙酰化/去乙酰化的表观遗传调控相关联。这种级联反应表明，病原体和宿主的共同进化可能涉及一系列复杂的攻击和反击，这些攻击和反击是基于入侵的真菌病原体及其昆虫宿主之间的通信。这一过程的解决决定了发病机制是否成功。
Parasitic fungi are the only pathogens that can infect insect hosts directly through their proteinaceous exoskeleton. Penetration of the cuticle requires the release of fungal enzymes, including proteinases, which act as virulence factors. Insects can sense fungal infections and activate innate immune responses, including the synthesis of antifungal peptides and proteinase inhibitors that neutralize the incoming proteinases. This well-studied host response is epigenetically regulated by histone acetylation/deacetylation. Here we show that entomopathogenic fungi can in turn sense the presence of insect-derived antifungal peptides and proteinase inhibitors, and respond by inducing the synthesis of chymotrypsin-like proteinases and metalloproteinases that degrade the host-derived defense molecules. The rapidity of this response is dependent on the virulence of the fungal strain. We confirmed the specificity of the pathogen response to host-derived defense molecules by LC/MS and RT-PCR analysis, and correlated this process with the epigenetic regulation of histone acetylation/deacetylation. This cascade of responses reveals that the coevolution of pathogens and hosts can involve a complex series of attacks and counterattacks based on communication between the invading fungal pathogen and its insect host. The resolution of this process determines whether or not pathogenesis is successful.
登录
查看更多内容
DOI：
10.1073/pnas.0912427107
发表时间：
2010-01-26
影响因子：
11.1
作者：
da Rosa, Jessica Lopes;Boyartchuk, Victor L.;Kaufman, Paul D.
通讯作者：
Kaufman, Paul D.
影响因子：
3.4
作者：
de Bekker C;Quevillon LE;Smith PB;Fleming KR;Ghosh D;Patterson AD;Hughes DP
通讯作者：
Hughes DP
影响因子：
6.7
作者：
Chaal BK;Gupta AP;Wastuwidyaningtyas BD;Luah YH;Bozdech Z
通讯作者：
Bozdech Z
影响因子：
4.4
作者：
Mukherjee K;Vilcinskas A
通讯作者：
Vilcinskas A
影响因子：
3.1
作者：
Altincicek, Boran;Linder, Monica;Vilcinskas, Andreas
通讯作者：
Vilcinskas, Andreas