Stimulation of erythrocyte cell membrane scrambling by mushroom tyrosinase.
Stimulation of erythrocyte cell membrane scrambling by mushroom tyrosinase.
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DOI：
10.3390/toxins6031096
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发表时间：
2014-03-18
期刊：
影响因子：
4.2
通讯作者：
Lang F
中科院分区：
文献类型：
--
作者：
Frauenfeld L;Alzoubi K;Abed M;Lang F
背景：蘑菇酪氨酸酶是一种含铜的酶，可调节肿瘤细胞的生长和存活。蘑菇酪氨酸酶可能促进细胞凋亡，部分原因是干扰线粒体功能。红细胞缺乏线粒体，但能够经历红细胞凋亡样自杀性细胞死亡或红细胞凋亡，其特征在于细胞收缩和细胞膜混乱，导致红细胞表面的磷脂酰丝氨酸暴露。细胞凋亡的触发信号包括胞质Ca 2+活性（[Ca 2 +]i）的增加和鞘磷脂酶的激活，随后形成神经酰胺。本研究探讨了酪氨酸酶是否具有促凋亡作用。研究方法：细胞体积已经从前向散射、膜联蛋白V结合的磷脂酰丝氨酸暴露、Fluo 3荧光的[Ca 2 +]i和流式细胞术中荧光抗体结合的神经酰胺丰度估计。结果如下：蘑菇酪氨酸酶（7 U/mL）暴露24 h后，[Ca 2 +]i显著增加，神经酰胺丰度显著增加，膜联蛋白V结合显著增加。酪氨酸酶处理后的膜联蛋白-V-结合显着钝化，但在细胞外Ca 2+的名义情况下没有废除。酪氨酸酶没有显着改变前向散射。结论：酪氨酸酶触发细胞膜混乱，效果，至少部分，由于进入细胞外Ca 2+和神经酰胺的形成。
Background: Mushroom tyrosinase, a copper containing enzyme, modifies growth and survival of tumor cells. Mushroom tyrosinase may foster apoptosis, an effect in part due to interference with mitochondrial function. Erythrocytes lack mitochondria but are able to undergo apoptosis-like suicidal cell death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling leading to phosphatidylserine-exposure at the erythrocyte surface. Signaling involved in the triggering of eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i) and activation of sphingomyelinase with subsequent formation of ceramide. The present study explored, whether tyrosinase stimulates eryptosis. Methods: Cell volume has been estimated from forward scatter, phosphatidylserine-exposure from annexin V binding, [Ca2+]i from Fluo3-fluorescence, and ceramide abundance from binding of fluorescent antibodies in flow cytometry. Results: A 24 h exposure to mushroom tyrosinase (7 U/mL) was followed by a significant increase of [Ca2+]i, a significant increase of ceramide abundance, and a significant increase of annexin-V-binding. The annexin-V-binding following tyrosinase treatment was significantly blunted but not abrogated in the nominal absence of extracellular Ca2+. Tyrosinase did not significantly modify forward scatter. Conclusions: Tyrosinase triggers cell membrane scrambling, an effect, at least partially, due to entry of extracellular Ca2+ and ceramide formation.
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影响因子：
--
作者：
Bissinger, Rosi;Modicano, Paola;Lang, Florian
通讯作者：
Lang, Florian
影响因子：
4.5
作者：
Abed, Majed;Towhid, Syeda T.;Lang, Florian
通讯作者：
Lang, Florian
影响因子：
3.7
作者：
Böttger E;Multhoff G;Kun JF;Esen M
通讯作者：
Esen M
影响因子：
4.5
作者：
Birka, C;Lang, PA;Lang, F
通讯作者：
Lang, F
影响因子：
6.1
作者：
Gao, Minghui;Cheung, K. L.;Kong, S. K.
通讯作者：
Kong, S. K.