Tube formation by complex cellular processes in Ciona intestinalis notochord.
Tube formation by complex cellular processes in Ciona intestinalis notochord.
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DOI：
10.1016/j.ydbio.2009.03.015
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发表时间：
2009-06-15
影响因子：
2.7
通讯作者：
Jiang, Di
中科院分区：
文献类型：
--
作者：
Dong, Bo;Horie, Takeo;Denker, Elsa;Kusakabe, Takehiro;Tsuda, Motoyuki;Smith, William C.;Jiang, Di
关键词：
在胚胎发生过程中，多细胞结构和器官由组成细胞组装而成。许多器官的一个共同的结构组成部分是管，它最简单的是由单层上皮细胞包围的管腔空间组成。海鞘玻璃海鞘的脊索形成一个只有40个细胞组成的管，并作为一个流体静力学的“骨架”游泳必不可少。虽然海鞘脊索发育中的会聚延伸的早期过程已被广泛研究，但发育的后期阶段，包括管腔形成，尚未得到很好的表征。在这里，我们使用分子标记和共聚焦成像来描述在发展中的玻璃海鞘脊索tubulogenesis。我们发现，在tubulogenesis每个脊索细胞建立从头顶端域，并经历了间充质上皮过渡，成为一个不寻常的上皮细胞与两个相反的顶端域。同时，细胞外腔基质的产生和沉积之间的脊索细胞。随后，每个脊索细胞同时在脊索鞘内表面沿前后轴沿着双向爬行。片状伪足样突起导致细胞沿前后轴沿着延长，而同一细胞后缘的回缩导致两个顶端区域的合并。结果，脊索细胞获得内皮样形状，并形成中央腔的壁。肌动蛋白聚合的抑制阻止了细胞的运动和管的形成。玻璃海鞘脊索管的形成利用了各种常见的和基本的细胞过程，包括细胞形状变化，顶端膜生物发生，细胞/细胞粘附重塑，动态细胞爬行，和管腔基质分泌。
In the course of embryogenesis multicellular structures and organs are assembled from constituent cells. One structural component common to many organs is the tube, which consists most simply of a luminal space surrounded by a single layer of epithelial cells. The notochord of ascidian Ciona forms a tube consisting of only 40 cells, and serves as a hydrostatic “skeleton” essential for swimming. While the early processes of convergent extension in ascidian notochord development have been extensively studied, the later phases of development, which include lumen formation, have not been well characterized. Here we used molecular markers and confocal imaging to describe tubulogenesis in the developing Ciona notochord. We found that during tubulogenesis each notochord cell established de novo apical domains, and underwent a mesenchymal-epithelial transition to become an unusual epithelial cell with two opposing apical domains. Concomitantly, extracellular luminal matrix was produced and deposited between notochord cells. Subsequently, each notochord cell simultaneously executed two types of crawling movements bi-directionally along the anterior/posterior axis on the inner surface of notochordal sheath. Lamellipodia-like protrusions resulted in cell lengthening along the anterior/posterior axis, while the retraction of trailing edges of the same cell led to the merging of the two apical domains. As a result, the notochord cells acquired endothelial-like shape and formed the wall of the central lumen. Inhibition of actin polymerization prevented the cell movement and tube formation. Ciona notochord tube formation utilized an assortment of common and fundamental cellular processes including cell shape change, apical membrane biogenesis, cell/cell adhesion remodeling, dynamic cell crawling, and lumen matrix secretion.
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