Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel.
Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel.
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DOI：
10.1186/1471-2164-13-507
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发表时间：
2012-09-25
期刊：
影响因子：
4.4
通讯作者：
Zane L
中科院分区：
文献类型：
--
作者：
Pujolar JM;Marino IA;Milan M;Coppe A;Maes GE;Capoccioni F;Ciccotti E;Bervoets L;Covaci A;Belpaire C;Cramb G;Patarnello T;Bargelloni L;Bortoluzzi S;Zane L
基因组和转录组方法有可能揭示全基因组对环境扰动的反应。自20世纪80年代以来，欧洲鳗鱼种群的数量一直在下降，可能是由于人为和气候因素的共同作用。在这篇文章中，我们探索了来自高污染环境(意大利台伯河)和低污染环境(意大利博尔塞纳湖)的个体之间的转录动态，对36种多氯联苯、几种有机氯农药和溴化阻燃剂以及9种金属进行了测量。为此，我们首先(I)使用深度测序数据更新了欧洲鳗鱼转录组，总共有640,040个读物组装成44,896个重叠群(Eeelbase Release 2.0)，以及(Ii)建立了一个转录转录平台，用于在极度濒危的欧洲鳗鱼中进行全球基因表达谱分析，该平台包含大约15,000个注释重叠群，用于检测污染部位之间的差异表达基因。几个与污染物代谢相关的解毒基因在高污染区表达上调，包括参与异源代谢第一阶段(CyP3A)、第二阶段(谷胱甘肽-S-转移酶)和氧化应激(谷胱甘肽过氧化物酶)的基因。此外，线粒体呼吸链和氧化磷酸化的关键基因在Tiber位点相对于Bolsena位点下调。与解毒基因的诱导高表达一起，推测参与新陈代谢的基因的低表达表明，污染也可能与呼吸和能量产生的减少有关。
Genomic and transcriptomic approaches have the potential for unveiling the genome-wide response to environmental perturbations. The abundance of the catadromous European eel (Anguilla anguilla) stock has been declining since the 1980s probably due to a combination of anthropogenic and climatic factors. In this paper, we explore the transcriptomic dynamics between individuals from high (river Tiber, Italy) and low pollution (lake Bolsena, Italy) environments, which were measured for 36 PCBs, several organochlorine pesticides and brominated flame retardants and nine metals. To this end, we first (i) updated the European eel transcriptome using deep sequencing data with a total of 640,040 reads assembled into 44,896 contigs (Eeelbase release 2.0), and (ii) developed a transcriptomic platform for global gene expression profiling in the critically endangered European eel of about 15,000 annotated contigs, which was applied to detect differentially expressed genes between polluted sites. Several detoxification genes related to metabolism of pollutants were upregulated in the highly polluted site, including genes that take part in phase I of the xenobiotic metabolism (CYP3A), phase II (glutathione-S-transferase) and oxidative stress (glutathione peroxidase). In addition, key genes in the mitochondrial respiratory chain and oxidative phosphorylation were down-regulated at the Tiber site relative to the Bolsena site. Together with the induced high expression of detoxification genes, the suggested lowered expression of genes supposedly involved in metabolism suggests that pollution may also be associated with decreased respiratory and energy production.
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影响因子：
56.9
作者：
Kessler, A;Halitschke, R;Baldwin, IT
通讯作者：
Baldwin, IT
影响因子：
4.1
作者：
Bozinovic, Goran;Oleksiak, Marjorie F.
通讯作者：
Oleksiak, Marjorie F.
影响因子：
8.9
作者：
Bervoets, L;Knaepkens, G;Blust, R
通讯作者：
Blust, R
DOI：
10.4415/ann_11_03_12
发表时间：
2011-01-01
期刊：
Annali dell'Istituto Superiore di Sanità
影响因子：
--
作者：
Miniero, Roberto;Guandalini, Emilio;De Felip, Elena
通讯作者：
De Felip, Elena
影响因子：
2.6
作者：
Bonhommeau, Sylvain;Chassot, Emmanuel;Rivot, Etienne
通讯作者：
Rivot, Etienne