Aerobic proteobacterial methylotrophs in Movile Cave: genomic and metagenomic analyses.
Aerobic proteobacterial methylotrophs in Movile Cave: genomic and metagenomic analyses.
复制标题
DOI：
10.1186/s40168-017-0383-2
复制
发表时间：
2018-01-02
期刊：
影响因子：
15.5
通讯作者：
Murrell JC
中科院分区：
文献类型：
--
作者：
Kumaresan D;Stephenson J;Doxey AC;Bandukwala H;Brooks E;Hillebrand-Voiculescu A;Whiteley AS;Murrell JC
Movile Cave（罗马尼亚Mangalia）是一个独特的生态系统，食物网由微生物初级生产维持，类似于深海热液喷口。具体地说，从硫化氢和甲烷的氧化中获得能量的化能自养微生物形成了食物网的基础。在这里，我们报告的第一个甲烷氧化细菌的分离从Movile洞穴生态系统中，Escheridatus甲基单胞菌属LWB，一个新的物种和代表Movile洞穴微生物垫样品。虽然以前的研究表明，缺氧条件下，在较深的湖水和沉积物的流行，使用小规模的鸟枪宏基因组测序，我们表明，代谢基因编码的酶有氧甲基营养是普遍存在于沉积物宏基因组可能表明存在微氧条件。此外，这项研究还表明，家庭内的成员Gallionellaceae（Sideroxydans和Gallionella）的沉积物微生物群落内的优势类群，从而表明微需氧铁氧化细菌在营养循环的Movile洞穴沉积物中的重要作用。在这项研究中，基于宏基因组序列的系统发育和代谢基因调查，有氧微生物过程（即，甲基营养和铁氧化）。我们还强调了我们对Movile Cave生态系统内生物地球化学循环的知识存在重大差距，以及需要进一步研究湖泊沉积物和湖水中微生物群落之间的潜在反馈机制。本文的在线版本（10.1186/s40168-017-0383-2）包含补充材料，可供授权用户使用。
Movile Cave (Mangalia, Romania) is a unique ecosystem where the food web is sustained by microbial primary production, analogous to deep-sea hydrothermal vents. Specifically, chemoautotrophic microbes deriving energy from the oxidation of hydrogen sulphide and methane form the basis of the food web. Here, we report the isolation of the first methane-oxidizing bacterium from the Movile Cave ecosystem, Candidatus Methylomonas sp. LWB, a new species and representative of Movile Cave microbial mat samples. While previous research has suggested a prevalence of anoxic conditions in deeper lake water and sediment, using small-scale shotgun metagenome sequencing, we show that metabolic genes encoding enzymes for aerobic methylotrophy are prevalent in sediment metagenomes possibly indicating the presence of microoxic conditions. Moreover, this study also indicates that members within the family Gallionellaceae (Sideroxydans and Gallionella) were the dominant taxa within the sediment microbial community, thus suggesting a major role for microaerophilic iron-oxidising bacteria in nutrient cycling within the Movile Cave sediments. In this study, based on phylogenetic and metabolic gene surveys of metagenome sequences, the possibility of aerobic microbial processes (i.e., methylotrophy and iron oxidation) within the sediment is indicated. We also highlight significant gaps in our knowledge on biogeochemical cycles within the Movile Cave ecosystem, and the need to further investigate potential feedback mechanisms between microbial communities in both lake sediment and lake water. The online version of this article (10.1186/s40168-017-0383-2) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
56.9
作者：
Sarbu, SM;Kane, TC;Kinkle, BK
通讯作者：
Kinkle, BK
影响因子：
14.9
作者：
Mitchell A;Bucchini F;Cochrane G;Denise H;ten Hoopen P;Fraser M;Pesseat S;Potter S;Scheremetjew M;Sterk P;Finn RD
通讯作者：
Finn RD
影响因子：
2.7
作者：
Darling AE;Jospin G;Lowe E;Matsen FA 4th;Bik HM;Eisen JA
通讯作者：
Eisen JA
影响因子：
5.1
作者：
Hutchens, E;Radajewski, S;Murrell, JC
通讯作者：
Murrell, JC
影响因子：
4.5
作者：
Muschiol, Daniel;Giere, Olav;Traunspurger, Walter
通讯作者：
Traunspurger, Walter