The effect of Ethylene Oxide Sterilisation, Beta Irradiation and Gamma Irradiation on Collagen Fibril-Based Scaffolds.
The effect of Ethylene Oxide Sterilisation, Beta Irradiation and Gamma Irradiation on Collagen Fibril-Based Scaffolds.
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环氧乙烷灭菌、β 辐射和伽马辐射对胶原纤维支架的影响。
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
T. Kuppevelt
中科院分区：
文献类型：
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作者：
K. A. Faraj;K. M. Brouwer;P. Geutjes;E. Versteeg;P. Wismans;J. Deprest;H. Chajra;D. M. Tiemissen;W. Feitz;E. Oosterwijk;W. Daamen;T. Kuppevelt
环氧乙烷(ETO)放气和β-和γ-辐射目前被用于胶原支架的灭菌。在此过程中，支架可能会发生化学和物理变化，这可能会损害其结构完整性和功能特性。在这项研究中，我们比较了15和25kGyETO放气以及β和γ照射对I型胶原纤维支架的影响，包括交联剂和不交联剂，以及肝素和不加肝素。使用广泛的生物物理、生化、形态和生物学参数进行评估。ETO处理、β照射和γ照射均未引起支架的形态改变，也没有影响支架上伯胺基团的数量或肝素的共价结合数量。细胞相容性也没有受到影响。然而，辐射确实导致胶原降解产物，胶原变性温度降低，蛋白降解增加，所有这些都是剂量依赖的方式。这些参数不受ETO处理的影响。灭菌方法对交联型支架的拉伸强度几乎没有影响，但令人惊讶的是，它们提高了非交联型支架的抗拉强度。结论：灭菌对胶原支架的一些参数有影响，而对其他参数没有影响。辐射的影响比ETO大得多。然而，抗张强度和细胞相容性在组织工程中很重要，任何一种方法都没有对其产生负面影响。因此，在选择灭菌方法时，可以考虑成本、安全性和实用性等方面的因素。
Ethylene oxide (EtO) gassing and β- and γ-irradiation are currently used for sterilising collagen scaffolds. During the process, scaffolds may undergo chemical and physical alterations that may compromise their structural integrity and functional characteristics. In this study, we compared the effects of EtO gassing, and β- and γ-irradia- tion at 15 and 25 kGy on type I collagen fibril-based scaffolds with and without crosslinking, and with and without heparin. Evaluation was performed using a wide range of biophysical, biochemical, morphological and biological parameters. EtO treatment, β-irradiation and γ-irradiation did not induce morphological changes, nor did they have an effect on the amount of primary amine groups, or the amount of heparin covalently attached to the scaffolds. Cyto- compatibility was also not affected. Irradiation, however, did result in collagen degradation products, a decrease in collagen denaturation temperature, and an increase in proteolytic degradation, all in a dose dependent fashion. These parameters were hardly influenced by EtO treatment. Sterilisation methods had hardly any effect on tensile strength of crosslinked scaffolds, but -surprisingly- they increased the tensile strength of non-crosslinked scaffolds. In con- clusion, a number of the collagen scaffold parameters were influenced by sterilisation, whereas others were not. Irra- diation had a much larger effect than EtO. However, tensile strength and cytocompatibility, important in tissue engineering, were not negatively influenced by any of the methods. Therefore, aspects like costs, safety and practi- cality of use may be taken into account in the choice of sterilisation method.
葡萄糖可稳定经伽马射线照射灭菌的胶原蛋白。
DOI：
10.1002/jbm.a.20018
发表时间：
2003
期刊：
Journal of biomedical materials research. Part A.
影响因子：
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作者：
Ohan,MarkP;Dunn,MichaelG
通讯作者：
Dunn,MichaelG