Plasma microRNA panel for minimally invasive detection of breast cancer.
Plasma microRNA panel for minimally invasive detection of breast cancer.
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DOI：
10.1371/journal.pone.0076729
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Burwinkel B
中科院分区：
文献类型：
--
作者：
Cuk K;Zucknick M;Madhavan D;Schott S;Golatta M;Heil J;Marmé F;Turchinovich A;Sinn P;Sohn C;Junkermann H;Schneeweiss A;Burwinkel B
在过去的几年中，循环 microRNA (miRNA) 已成为各种疾病（包括癌症）的有前途的新型微创标记物。我们已经表明，与健康女性相比，乳腺癌患者血浆中的某些 miRNA 失调。在此，我们进一步探索了它们作为血浆中乳腺癌早期检测标记物的潜力。从基于 miRNA 阵列的筛选中选出的候选 miR-127-3p、miR-376a 和 miR-652 首次被发现与乳腺癌相关 (n = 417)。此外，我们在一个独立队列 (n = 210) 中验证了之前报道的循环 miRNA（miR-148b、miR-376c、miR-409-3p 和 miR-801），与健康女性相比，乳腺癌患者血浆中的循环 miRNA 升高。我们首次在乳腺癌中描述了源自染色体 14q32 区域的失调 miRNA（miR-127-3p、miR-376a、miR-376c 和 miR-409-3p）的过度表达。通过纳入良性乳腺肿瘤患者，我们可以观察到，与健康对照相比，良性肿瘤女性血浆中的 miR-148b、miR-652 和 miR-801 水平甚至升高。此外，对按癌症分期分层的样本进行分析表明，miR-127-3p、miR-148b、miR-409-3p、miR-652 和 miR-801 还可以检测 I 期或 II 期乳腺癌，从而使其成为早期检测的有吸引力的候选者。最后，ROC 曲线分析显示，这 7 个循环 miRNA 组成的一组对于良性和恶性乳腺肿瘤的检测具有很大的诊断潜力，AUC 为 0.81，在年轻女性（50 岁以下）中进一步增加到 0.86。
Over the last few years, circulating microRNAs (miRNAs) have emerged as promising novel and minimally invasive markers for various diseases, including cancer. We already showed that certain miRNAs are deregulated in the plasma of breast cancer patients when compared to healthy women. Herein we have further explored their potential to serve as breast cancer early detection markers in blood plasma. Circulating miR-127-3p, miR-376a and miR-652, selected as candidates from a miRNA array-based screening, were found to be associated with breast cancer for the first time (n = 417). Further we validated our previously reported circulating miRNAs (miR-148b, miR-376c, miR-409-3p and miR-801) in an independent cohort (n = 210) as elevated in the plasma of breast cancer patients compared to healthy women. We described, for the first time in breast cancer, an over-representation of deregulated miRNAs (miR-127-3p, miR-376a, miR-376c and miR-409-3p) originating from the chromosome 14q32 region. The inclusion of patients with benign breast tumors enabled the observation that miR-148b, miR-652 and miR-801 levels are even elevated in the plasma of women with benign tumors when compared to healthy controls. Furthermore, an analysis of samples stratified by cancer stage demonstrated that miR-127-3p, miR-148b, miR-409-3p, miR-652 and miR-801 can detect also stage I or stage II breast cancer thus making them attractive candidates for early detection. Finally, ROC curve analysis showed that a panel of these seven circulating miRNAs has substantial diagnostic potential with an AUC of 0.81 for the detection of benign and malignant breast tumors, which further increased to 0.86 in younger women (up to 50 years of age).
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