Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal.
Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal.
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DOI：
10.1038/srep37595
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发表时间：
2016-11-24
影响因子：
4.6
通讯作者：
Sode K
中科院分区：
文献类型：
--
作者：
Nakajima M;Ferri S;Rögner M;Sode K
蓝细菌拥有独特的光感受器，被称为蓝细菌色素（CBCRs）。在这项研究中，我们试图设计一种来自蓝细菌Synechocystis sp. PCC 6803的彩色驯化传感器CcaS。野生型CcaS在绿光照射下诱导基因表达，在红光照射下抑制基因表达。我们重点研究了CcaS的结构域结构，它包括一个n端跨膜螺旋；GAF域作为传感器域；连接区域（L1）；两个PAS域；第二连接器区域（L2）；c端组氨酸激酶（HK）结构域。通过去除L1连接区域和两个PAS结构域，并用截断的连接区域融合GAF和HK结构域，构建了截断版的光感受器。因此，根据对绿光和红光的响应，将构建的“小型化中央花楸”分为四种不同的类别，其中一些与野生型相比表现出更好的基因调控。值得注意的是，其中一个小型化的CcaSs在红光下诱导基因表达，在绿光下抑制基因表达，与野生型CcaS相比，这是对光信号的相反反应。通过对CcaS结构模型的分析，讨论了工程光感受器的这些特性。
Cyanobacteria harbor unique photoreceptors, designated as cyanobacteriochromes (CBCRs). In this study, we attempted to engineer the chromatic acclimation sensor CcaS, a CBCR derived from the cyanobacterium Synechocystis sp. PCC 6803. The wild-type CcaS induces gene expression under green light illumination and represses it under red light illumination. We focused on the domain structure of CcaS, which consists of an N-terminal transmembrane helix; a GAF domain, which serves as the sensor domain; a linker region (L1); two PAS domains; a second linker region (L2); and a C-terminal histidine kinase (HK) domain. Truncated versions of the photoreceptor were constructed by removing the L1 linker region and the two PAS domains, and fusing the GAF and HK domains with a truncated linker region. Thus constructed “miniaturized CcaSs” were grouped into four distinct categories according to their responses toward green and red light illumination, with some showing improved gene regulation compared to the wild type. Remarkably, one of the miniaturized CcaSs induced gene expression under red light and repressed it under green light, a reversed response to the light signal compared to wild type CcaS. These characteristics of engineered photoreceptors were discussed by analyzing the CcaS structural model.
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