Trophic Status Is Associated With Community Structure and Metabolic Potential of Planktonic Microbiota in Plateau Lakes
Trophic Status Is Associated With Community Structure and Metabolic Potential of Planktonic Microbiota in Plateau Lakes
复制标题
高原湖泊浮游微生物群落结构和代谢潜力与营养状况相关
DOI：
10.3389/fmicb.2019.02560
复制
发表时间：
2019-11
影响因子：
5.2
通讯作者：
Zhao Jindong
中科院分区：
文献类型：
--
作者：
Shen Mengyuan;Li Qi;Ren Minglei;Lin Yan;Wang Juanping;Chen Li;Li Tao;Zhao Jindong
微生物在各种水生生态系统中对全球能量通量和生态地球化学过程起着关键作用。然而，不同营养状态的淡水湖泊中微生物群落的功能结构和代谢潜力的详细模式仍有待了解。我们采用宏基因组学的工作流程来分析营养状态和浮游微生物群在云贵高原，中国淡水湖泊之间的相关性。结果表明，富营养化和中营养-贫营养湖泊生态系统中的微生物群落具有不同的群落结构和代谢潜力。蓝藻在富营养化生态系统中占主导地位，主要驱动该生态系统群的好氧呼吸、发酵、氮同化、氮矿化、同化硫酸盐还原和硫矿化等过程。放线菌、变形菌（α-、β-和γ-变形菌）、疣微菌和浮游菌在中营养-贫营养生态系统中的出现频率高于富营养生态系统，这些类群可能介导上述代谢过程。在这两组生态系统中，参与碳水化合物代谢、能量代谢、聚糖生物合成和代谢以及辅因子和维生素代谢的功能基因丰度的差异显著有助于地表水微生物群的独特功能结构。此外，微生物介导的碳，氮和硫转化的代谢潜力在两个生态系统组中表现出差异。与中营养-贫营养生态系统相比，富营养生态系统中的厌氧微生物群落具有更高的好氧固碳、发酵、产甲烷、厌氧氨氧化、反硝化和硫矿化能力，但其好氧呼吸、CO氧化、固氮和同化硫酸盐还原能力较低。这项研究提供了深入了解营养状态的关系，浮游微生物群落结构及其代谢潜力，并确定了负责淡水湖泊环境中的碳，氮和硫的生态地球化学循环的主要类群。
Microbes in various aquatic ecosystems play a key role in global energy fluxes and biogeochemical processes. However, the detailed patterns on the functional structure and the metabolic potential of microbial communities in freshwater lakes with different trophic status remain to be understood. We employed a metagenomics workflow to analyze the correlations between trophic status and planktonic microbiota in freshwater lakes on Yun-Gui Plateau, China. Our results revealed that microbial communities in the eutrophic and mesotrophic-oligotrophic lake ecosystems harbor distinct community structure and metabolic potential. Cyanobacteria were dominant in the eutrophic ecosystems, mainly driving the processes of aerobic respiration, fermentation, nitrogen assimilation, nitrogen mineralization, assimilatory sulfate reduction and sulfur mineralization in this ecosystem group. Actinobacteria, Proteobacteria (Alpha-, Beta-, and Gammaproteobacteria), Verrucomicrobia and Planctomycetes, occurred more often in the mesotrophic-oligotrophic ecosystems than those in the eutrophic ecosystems, and these taxa potentially mediate the above metabolic processes. In these two groups of ecosystems, a difference in the abundance of functional genes involved in carbohydrate metabolism, energy metabolism, glycan biosynthesis and metabolism, and metabolism of cofactors and vitamins significantly contribute to the distinct functional structure of microbiota from surface water. Furthermore, the microbe-mediated metabolic potentials for carbon, nitrogen and sulfur transformation showed differences in the two ecosystem groups. Compared with the mesotrophic-oligotrophic ecosystems, planktonic microbial communities in the eutrophic ecosystems showed higher potential for aerobic carbon fixation, fermentation, methanogenesis, anammox, denitrification, and sulfur mineralization, but they showed lower potential for aerobic respiration, CO oxidation, nitrogen fixation, and assimilatory sulfate reduction. This study offers insights into the relationships of trophic status to planktonic microbial community structure and its metabolic potential, and identifies the main taxa responsible for the biogeochemical cycles of carbon, nitrogen and sulfur in freshwater lake environments.
登录
查看更多内容
影响因子：
15.5
作者：
Arora-Williams K;Olesen SW;Scandella BP;Delwiche K;Spencer SJ;Myers EM;Abraham S;Sooklal A;Preheim SP
通讯作者：
Preheim SP
影响因子：
15.5
作者：
Vavourakis CD;Andrei AS;Mehrshad M;Ghai R;Sorokin DY;Muyzer G
通讯作者：
Muyzer G
影响因子：
2.7
作者：
Castro-Nallar E;Bendall ML;Pérez-Losada M;Sabuncyan S;Severance EG;Dickerson FB;Schroeder JR;Yolken RH;Crandall KA
通讯作者：
Crandall KA
太湖蓝藻水华期蓝藻聚集体的宏转录组学分析
DOI：
10.1007/s11356-017-0733-4
发表时间：
2018-02
期刊：
Environ Sci Pollut Res
影响因子：
--
作者：
Junyi Zhang
通讯作者：
Junyi Zhang
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.