Prostaglandin E2 cooperates with TRANCE in osteoclast induction from hemopoietic precursors: synergistic activation of differentiation, cell spreading, and fusion.
Prostaglandin E2 cooperates with TRANCE in osteoclast induction from hemopoietic precursors: synergistic activation of differentiation, cell spreading, and fusion.
复制标题
前列腺素 E2 与 TRANCE 合作，诱导造血前体细胞诱导破骨细胞：协同激活分化、细胞扩散和融合。
作者：
M. Wani;K. Fuller;N. Kim;Yongwon Choi;Yongwon Choi;T. Chambers
最近发现，成骨细胞表达TRANCE（肿瘤坏死因子相关激活诱导细胞因子），这是肿瘤坏死因子超家族中新发现的成员，并且在嗜钙激素的作用下表达增加。此外，可溶性重组TRANCE诱导造血前体细胞无基质群体中破骨细胞的形成和吸收。然而，体内诱饵受体骨保护素的过表达表明，不同部位对吸收抑制的敏感性存在实质性差异，这表明要么存在替代配体，要么破骨细胞对TRANCE的敏感性可以通过辅因子进行修饰。因此，我们测试了辅助因子可能通过TRANCE促进破骨细胞形成的可能性。我们发现，在没有基质细胞的情况下，PGE2的存在使抗酒石酸酸性磷酸酶阳性和降钙素受体阳性细胞的数量增加了10倍。此外，尽管仅在TRANCE中形成的抗酒石酸酸性磷酸酶阳性细胞通常是单核的，扩散不良，但PGE2的加入诱导形成了大的，扩散良好的多核细胞。骨吸收的增加与破骨细胞数量的增加相对应。PGE2不与TRANCE协同刺激成熟细胞的吸收。8-Bromo-cAMP对破骨细胞分化具有类似的协同作用。因此，PGE2似乎通过对造血前体的直接作用来刺激骨吸收，主要是通过对TRANCE诱导破骨细胞分化能力的协同作用。
It was recently found that osteoblastic cells express TRANCE (tumor necrosis factor-related activation-induced cytokine), a newly identified member of the tumor necrosis factor superfamily, and that expression was increased by calciotropic hormones. Furthermore, soluble recombinant TRANCE induces osteoclast formation and resorption in stroma-free populations of hemopoietic precursor cells. However, overexpression of the decoy receptor osteoprotegerin in vivo shows that there are substantial differences in the sensitivity of different sites to resorption-inhibition, suggesting that either alternative ligands exist or the sensitivity of osteoclasts to TRANCE can be modified by cofactors. We therefore tested the possibility that cofactors might enhance osteoclast formation by TRANCE. We found that the number of tartrate-resistant acid phosphatase-positive and calcitonin receptor-positive cells was increased by a factor of 10 by the presence of PGE2 in the absence of stromal cells. Moreover, although the tartrate-resistant acid phosphatase-positive cells that formed in TRANCE alone were typically mononuclear and poorly spread, the addition of PGE2 induced the formation of large, well spread multinuclear cells. There was an increase in bone resorption that corresponded with the increase in osteoclast number. PGE2 did not synergize with TRANCE for resorption-stimulation in mature cells. 8-Bromo-cAMP showed a similar syngergistic effect on osteoclastic differentiation. Thus, PGE2 appears to stimulate bone resorption through a direct effect on hemopoietic precursors, primarily through a synergistic effect on the ability of TRANCE to induce osteoclastic differentiation.
α 和 β 人类转化生长因子刺激培养小鼠颅骨中前列腺素的产生和骨吸收。
DOI：
10.1073/pnas.82.13.4535
发表时间：
1985
影响因子：
11.1
作者：
TashjianJr,AH;Voelkel,EF;Lazzaro,M;Singer,FR;Roberts,AB;Derynck,R;Winkler,ME;Levine,L
通讯作者：
Levine,L
血小板衍生生长因子通过前列腺素介导的机制刺激骨吸收。
影响因子：
4.8
作者：
TashjianJr,AH;Hohmann,EL;Antoniades,HN;Levine,L
通讯作者：
Levine,L