Small ORFs as New Regulators of Pri-miRNAs and miRNAs Expression in Human and Drosophila.
Small ORFs as New Regulators of Pri-miRNAs and miRNAs Expression in Human and Drosophila.
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DOI：
10.3390/ijms23105764
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发表时间：
2022-05-20
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
MicroRNAs (miRNAs) are small regulatory non-coding RNAs, resulting from the cleavage of long primary transcripts (pri-miRNAs) in the nucleus by the Microprocessor complex generating precursors (pre-miRNAs) that are then exported to the cytoplasm and processed into mature miRNAs. Some miRNAs are hosted in pri-miRNAs annotated as long non-coding RNAs (lncRNAs) and defined as MIRHGs (for miRNA Host Genes). However, several lnc pri-miRNAs contain translatable small open reading frames (smORFs). If smORFs present within lncRNAs can encode functional small peptides, they can also constitute cis-regulatory elements involved in lncRNA decay. Here, we investigated the possible involvement of smORFs in the regulation of lnc pri-miRNAs in Human and Drosophila, focusing on pri-miRNAs previously shown to contain translatable smORFs. We show that smORFs regulate the expression levels of human pri-miR-155 and pri-miR-497, and Drosophila pri-miR-8 and pri-miR-14, and also affect the expression and activity of their associated miRNAs. This smORF-dependent regulation is independent of the nucleotidic and amino acidic sequences of the smORFs and is sensitive to the ribosome-stalling drug cycloheximide, suggesting the involvement of translational events. This study identifies smORFs as new cis-acting elements involved in the regulation of pri-miRNAs and miRNAs expression, in both Human and Drosophila melanogaster.
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DOI：
10.1261/rna.053561.115
发表时间：
2016-06
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Carlevaro-Fita J;Rahim A;Guigó R;Vardy LA;Johnson R
通讯作者：
Johnson R
影响因子：
--
作者：
Allas U;Liiv A;Remme J
通讯作者：
Remme J
影响因子：
2.7
作者：
Bolin K;Rachmaninoff N;Moncada K;Pula K;Kennell J;Buttitta L
通讯作者：
Buttitta L
影响因子：
4.5
作者：
Cai, XZ;Hagedorn, CH;Cullen, BR
通讯作者：
Cullen, BR
DOI：
10.3109/10409238.2015.1117054
发表时间：
2016-05
影响因子：
6.5
作者：
Hata A;Kashima R
通讯作者：
Kashima R