Postexercise phosphocreatine recovery, an index of mitochondrial oxidative phosphorylation, is reduced in diabetic patients with lower extremity complications.
Postexercise phosphocreatine recovery, an index of mitochondrial oxidative phosphorylation, is reduced in diabetic patients with lower extremity complications.
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DOI：
10.1016/j.jvs.2012.10.011
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发表时间：
2013-04
影响因子：
4.3
通讯作者：
Veves, Aristidis
中科院分区：
文献类型：
--
作者：
Tecilazich, Francesco;Thanh Dinh;Lyons, Thomas E.;Guest, Julie;Villafuerte, Rosemond A.;Sampanis, Christos;Gnardellis, Charalambos;Zuo, Chun S.;Veves, Aristidis
目的：确定有和无下肢并发症的糖尿病患者运动后磷酸肌酸(PCR)恢复的差异，这是线粒体功能的一项指标。我们招募了健康对照组和三组T2 DM患者：无并发症、有周围神经病变以及周围神经病变和周围动脉病变的患者。我们采用磁共振波谱(MRS)测量方法，在3分钟的小腿后肌(腓肠肌和比目鱼肌)水平进行连续的磷代谢产物(PCr和PI)的测量。还进行了微血管和大血管反应性测量。所有组的静息PI/PCR值、基线和运动时达到的最大PCR值相似。在糖尿病合并神经病变和既有神经病变又有PAD的患者中，运动后PI/PCR值和PCR值恢复到静息水平所需的时间(线粒体氧化磷酸化的评估)显著更长(P<；0.01)。两组患者的肿瘤坏死因子α和粒细胞集落刺激因子水平也较高(P<0.05)。多元回归分析显示，只有G-CSF、OPG和肿瘤坏死因子α是PI/PCR值恢复时间变化的显著影响因素。未观察到微血管和大血管反应性测量与PI/PCR值或PCR恢复期之间的关系。线粒体氧化磷酸化仅在有神经病变的T2 DM患者中受损，无论是否存在PAD，并与在这些组中观察到的促炎状态增加有关。
To identify differences in the post-exercise phosphocreatine (PCr) recovery, an index of mitochondrial function, in diabetic patients with and without lower extremity complications. We enrolled healthy control subjects and three groups of T2DM patients: patients without complications, with peripheral neuropathy and both peripheral neuropathy and peripheral arterial disease. We employed Magnetic Resonance Spectroscopic (MRS) measurements to perform continuous measurements of phosphorous metabolites (PCr and Pi) during a 3-minute graded exercise at the level of the posterior calf muscles (gastrocnemius and soleus muscles). Micro- and macrovascular reactivity measurements were also performed. The resting Pi/PCr ratio and PCr at baseline and the maximum reached during exercise was similar in all groups. The post-exercise time required for recovery of Pi/PCr ratio and PCr levels to resting levels, an assessment of mitochondrial oxidative phosphorylation, was significantly higher in the diabetic patients with neuropathy and those with both neuropathy and PAD (p <0.01 for both measurements). These two groups had also higher levels of TNFα, (p<0.01) and G-CSF (p <0.05). Multiple regression analysis showed that only G-CSF, OPG and TNFα were significant contributing factors in the variation of the Pi/PCr ratio recovery time. No associations were observed between micro- and macrovascular reactivity measurements and Pi/Pcr ration or Pcr recovery time. Mitochondrial oxidative phosphorylation is impaired only in T2DM patients with neuropathy whether PAD is present or not and is associated with the increased proinflammatory state that was observed in these groups.
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影响因子：
16.2
作者：
Dinh T;Doupis J;Lyons TE;Kuchibhotla S;Julliard W;Gnardellis C;Rosenblum BI;Wang X;Giurini JM;Greenman RL;Veves A
通讯作者：
Veves A
影响因子：
7.7
作者：
Karakelides, Helen;Asmann, Yan W.;Nair, K. Sreekumaran
通讯作者：
Nair, K. Sreekumaran
影响因子：
7.7
作者：
Caballero, AE;Arora, S;Veves, A
通讯作者：
Veves, A
影响因子：
5.8
作者：
Doupis, John;Lyons, Thomas E.;Veves, Aristidis
通讯作者：
Veves, Aristidis
影响因子：
4.3
作者：
Greiner, A;Esterhammer, R;Schocke, MFH
通讯作者：
Schocke, MFH