Therapeutic intraspinal microstimulation improves forelimb function after cervical contusion injury.
Therapeutic intraspinal microstimulation improves forelimb function after cervical contusion injury.
复制标题
DOI：
10.1088/1741-2560/10/4/044001
复制
发表时间：
2013-08
影响因子：
4
通讯作者：
Moritz CT
中科院分区：
文献类型：
--
作者：
Kasten MR;Sunshine MD;Secrist ES;Horner PJ;Moritz CT
椎管内微刺激（ISMS）是一种很有前途的方法，激活脊髓远端的损伤。本研究的目的是检查长期植入颈髓内的刺激导线（1）直接产生前肢运动的能力，以及（2）评估ISMS刺激是否改善损伤后麻痹肢体的后续意志控制。我们开发了一种在Long-Evans大鼠颈髓C6-T1节段内植入椎管内刺激电极的技术。在C4-C5处的严重颈部挫伤后4周开始，在治疗条件下的动物接受治疗性ISMS 7小时/天，5天/周，持续随后的12周。在12周的治疗ISMS，刺激诱发的前肢运动是相对稳定的。我们还探讨了治疗性ISMS是否促进前肢伸展运动的恢复。在没有刺激的情况下进行的行为测试期间，接受每日治疗性ISMS的动物的表现显著优于未受刺激的动物。对前肢运动的定量视频分析表明，受刺激的动物在通过刺激加强的运动中表现更好，包括伸展手肘以推进前肢和打开手指。虽然引起前肢运动的阈值电流随着时间的推移逐渐增加，但在长期刺激和未刺激的电极之间没有观察到差异，这表明电刺激不会产生额外的组织损伤。结果表明，通过颈髓内的慢性微导丝植入物提供的治疗性椎管内刺激提供的益处超出了刺激期，这表明了神经装置促进损伤后持续恢复的未来策略。
Intraspinal microstimulation (ISMS) is a promising method for activating the spinal cord distal to an injury. The objectives of this study were to examine the ability of chronically implanted stimulating wires within the cervical spinal cord to (1) directly produce forelimb movements, and (2) assess whether ISMS stimulation improved subsequent volitional control of paretic extremities following injury. We developed a technique for implanting intraspinal stimulating electrodes within the cervical spinal cord segments C6-T1 of Long-Evans rats. Beginning 4 weeks after a severe cervical contusion injury at C4–C5, animals in the treatment condition received therapeutic ISMS 7 hours/day, 5 days/week for the following 12 weeks. Over 12 weeks of therapeutic ISMS, stimulus-evoked forelimb movements were relatively stable. We also explored whether therapeutic ISMS promotes recovery of forelimb reaching movements. Animals receiving daily therapeutic ISMS performed significantly better than unstimulated animals during behavioral tests conducted without stimulation. Quantitative video analysis of forelimb movements showed that stimulated animals performed better in the movements reinforced by stimulation, including extending the elbow to advance the forelimb and opening the digits. While threshold current to elicit forelimb movement gradually increased over time, no differences were observed between chronically stimulated and unstimulated electrodes suggesting that no additional tissue damage was produced by the electrical stimulation. The results indicate that therapeutic intraspinal stimulation delivered via chronic microwire implants within the cervical spinal cord confers benefits extending beyond the period of stimulation, suggesting future strategies for neural devices to promote sustained recovery after injury.
登录
查看更多内容
影响因子：
5.3
作者：
Brus-Ramer, Marcel;Carmel, Jason B.;Martin, John H.
通讯作者：
Martin, John H.
影响因子：
5.3
作者：
Houle, John D.;Tom, Veronica J.;Silver, Jerry
通讯作者：
Silver, Jerry
DOI：
10.1523/jneurosci.1435-10.2010
发表时间：
2010-08-11
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Carmel JB;Berrol LJ;Brus-Ramer M;Martin JH
通讯作者：
Martin JH
影响因子：
4.2
作者：
Dobkin, B.;Barbeau, H.;Scott, M.
通讯作者：
Scott, M.
影响因子：
2.7
作者：
Alaverdashvili, Mariam;Leblond, Hugues;Whishaw, Ian Q.
通讯作者：
Whishaw, Ian Q.