Light-dependent expression of flg22-induced defense genes in Arabidopsis.
Light-dependent expression of flg22-induced defense genes in Arabidopsis.
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DOI：
10.3389/fpls.2014.00531
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发表时间：
2014
影响因子：
5.6
通讯作者：
Shiina T
中科院分区：
文献类型：
--
作者：
Sano S;Aoyama M;Nakai K;Shimotani K;Yamasaki K;Sato MH;Tojo D;Suwastika IN;Nomura H;Shiina T
据报道，叶绿体产生逆行免疫信号，激活细胞核中的防御基因表达。然而，光和光合作用在植物免疫中的作用在很大程度上仍然难以捉摸。在这项研究中，我们评估了光对flg 22诱导的防御基因表达的影响，flg 22是一种来自细菌鞭毛蛋白的肽，在植物中作为一种有效的激发子。在光照和黑暗条件下对flg 22处理的拟南芥幼苗进行30分钟的全转录组分析显示，许多（30%）被flg 22强烈诱导的基因（>4.0）需要光来快速表达，而flg 22抑制的基因包括大量被光下调的基因。此外，光是负责flg 22诱导的水杨酸（SA）的积累，表明光是必不可少的基础防御反应在植物中。为了阐明光合作用在防御中的作用，我们进一步研究了在光合电子传递的特异性抑制剂3-（3，4-二氯苯基）-1，1-二甲基脲（DCMU）和2，5-二溴-3-甲基-6-异丙基-苯醌（DBMIB）存在下flg 22诱导的防御基因表达。光依赖性的防御基因的表达在很大程度上受到抑制DBMIB，但只有部分抑制DCMU。这些发现表明，光合电子流在控制flg 22诱导的防御基因的光依赖性表达中起作用。
Chloroplasts have been reported to generate retrograde immune signals that activate defense gene expression in the nucleus. However, the roles of light and photosynthesis in plant immunity remain largely elusive. In this study, we evaluated the effects of light on the expression of defense genes induced by flg22, a peptide derived from bacterial flagellins which acts as a potent elicitor in plants. Whole-transcriptome analysis of flg22-treated Arabidopsis thaliana seedlings under light and dark conditions for 30 min revealed that a number of (30%) genes strongly induced by flg22 (>4.0) require light for their rapid expression, whereas flg22-repressed genes include a significant number of genes that are down-regulated by light. Furthermore, light is responsible for the flg22-induced accumulation of salicylic acid (SA), indicating that light is indispensable for basal defense responses in plants. To elucidate the role of photosynthesis in defense, we further examined flg22-induced defense gene expression in the presence of specific inhibitors of photosynthetic electron transport: 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-benzoquinone (DBMIB). Light-dependent expression of defense genes was largely suppressed by DBMIB, but only partially suppressed by DCMU. These findings suggest that photosynthetic electron flow plays a role in controlling the light-dependent expression of flg22-inducible defense genes.
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影响因子：
4.6
作者：
Lyons, Rebecca;Iwase, Akira;Gansewig, Thomas;Sherstnev, Alexander;Duc, Celine;Barton, Geoffrey J.;Hanada, Kousuke;Higuchi-Takeuchi, Mieko;Matsui, Minami;Sugimoto, Keiko;Kazan, Kemal;Simpson, Gordon G.;Shirasu, Ken
通讯作者：
Shirasu, Ken
影响因子：
3.5
作者：
Goehre, Vera;Jones, Alexandra M. E.;Weber, Andreas P. M.
通讯作者：
Weber, Andreas P. M.
影响因子：
11.6
作者：
Ishihama, Nobuaki;Yamada, Reiko;Yoshioka, Hirofumi
通讯作者：
Yoshioka, Hirofumi
影响因子：
64.8
作者：
Han, SC;Tang, RH;Pei, ZM
通讯作者：
Pei, ZM
DOI：
10.1073/pnas.1004529107
发表时间：
2010-07-27
影响因子：
11.1
作者：
Jeong, Rae-Dong;Chandra-Shekara, A. C.;Kachroo, Pradeep
通讯作者：
Kachroo, Pradeep