Alterations to the gut microbiome impair bone tissue strength in aged mice.
Alterations to the gut microbiome impair bone tissue strength in aged mice.
复制标题
肠道微生物组的改变会损害老年小鼠的骨组织强度。
DOI：
10.1016/j.bonr.2021.101065
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发表时间：
2021-06
期刊：
影响因子：
2.5
通讯作者：
Rowan S
中科院分区：
文献类型：
--
作者：
Castaneda M;Smith KM;Nixon JC;Hernandez CJ;Rowan S
全骨强度和抗骨折性是由骨量和骨质量的组合决定的-这是决定骨质疏松症和年龄相关性骨折风险的关键因素。最近的临床前研究表明，肠道微生物组的改变可以影响骨量和骨组织质量。之前关于肠道微生物组和骨骼的工作仅限于年轻动物，目前尚不清楚肠道微生物组是否可以改变老年动物的骨组织强度。在这里，我们询问肠道微生物组成分的改变是否会影响老年小鼠（12-24个月大）的骨强度。在标准食物饮食中饲养直至12个月大的雄性C57 BL/6 J小鼠被分配到三种饮食中的一种：高血糖、低血糖或含有抗生素（氨苄青霉素和新霉素）的低血糖饮食，以改变肠道微生物组的组成。喂食含抗生素的低血糖饮食的组显示出不能用几何学解释的全骨强度降低，表明骨组织强度降低（p < 0.007）。与低血糖组相比，高血糖饮食组具有更大的骨横截面积和转动惯量以及相应的更大的骨强度，但是组织强度与低血糖组没有显著差异。这些发现表明，改变老年小鼠的肠道微生物组可以改变骨组织质量。通过抗生素改变老年小鼠的肠道微生物组会降低骨组织强度。高血糖指数饮食导致体重和骨骼尺寸增加。抗生素导致微生物组的变化比膳食淀粉含量更大。
Whole bone strength and resistance to fracture are determined by a combination of bone quantity and bone quality – key factors in determining risk for osteoporosis and age-related fractures. Recent preclinical studies have shown that alterations to the gut microbiome can influence bone quantity as well as bone tissue quality. Prior work on the gut microbiome and bone has been limited to young animals, and it is unknown if the gut microbiome can alter bone tissue strength in aged animals. Here we ask if alterations to the constituents of the gut microbiome influence bone strength in older mice (12–24 months of age). Male C57BL/6J mice raised on a standard chow diet until 12 months of age were assigned to one of three diets: high glycemic, low glycemic, or low glycemic diet containing antibiotics (ampicillin and neomycin) to modify the constituents of the gut microbiome. The group fed the low glycemic diet containing antibiotics showed reductions in whole bone strength that could not be explained by geometry, indicating reduced bone tissue strength (p < 0.007). The high glycemic diet group had larger bone cross-sectional area and moment of inertia and a corresponding greater bone strength as compared to the low glycemic groups, however tissue strength did not noticeably differ from that of the low glycemic group. These findings demonstrate that modifying the gut microbiome in aged mice can alter bone tissue quality. Altering the gut microbiome of aged mice via antibiotics reduces bone tissue strength. High-glycemic index diet leads to increased body weight and bone size. Antibiotics lead to greater alterations in the microbiome than dietary starch content.
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影响因子：
6.2
作者：
Kopperdahl, David L.;Aspelund, Thor;Hoffmann, Paul F.;Sigurdsson, Sigurdur;Siggeirsdottir, Kristin;Harris, Tamara B.;Gudnason, Vilmundur;Keaveny, Tony M.
通讯作者：
Keaveny, Tony M.
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
64.8
作者：
Claesson, Marcus J.;Jeffery, Ian B.;O'Toole, Paul W.
通讯作者：
O'Toole, Paul W.
影响因子：
4.3
作者：
Alliston, Tamara
通讯作者：
Alliston, Tamara
DOI：
10.1002/jbmr.2539
发表时间：
2015-06
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Jepsen KJ;Silva MJ;Vashishth D;Guo XE;van der Meulen MC
通讯作者：
van der Meulen MC