Guinea pig model of Mycobacterium tuberculosis latent/dormant infection.
Guinea pig model of Mycobacterium tuberculosis latent/dormant infection.
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DOI：
10.1016/j.micinf.2008.08.010
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发表时间：
2008-11
影响因子：
5.8
通讯作者：
Campos-Neto A
中科院分区：
文献类型：
--
作者：
Kashino SS;Napolitano DR;Skobe Z;Campos-Neto A
Most experimental studies of latent or dormant tuberculosis infection have been conducted in murine models. Although guinea pigs are considered one of the animals that best reproduce human tuberculosis, surprisingly little data exist describing latent or dormant infection in these animals. The present work addresses this issue and shows that guinea pigs infected with a streptomycin auxotrophic mutant of Mycobacterium tuberculosis (strain 18b) replicate most of the known clinical, immunological, and pathological manifestations of this phase of the infectious process in humans. To establish infection, animals were inoculated with the mutant followed by administration of streptomycin for three weeks. Withdrawal of streptomycin caused microbial dormancy and few microorganisms remained viable and could be recovered from the animals’ lungs and spleen several months later. Guinea pigs with dormant infection steadily gained weight and presented no clinical signs (scuff fur and lethargy) of active disease. The histopathology of the tuberculous lesions mimicked human lesions well. In addition, PBMC from infected animals strongly responded to stimulation with PPD or soluble culture filtrate proteins of M. tuberculosis throughout the duration of the experiment (six months). Finally, the tuberculin skin test (a clinical hallmark of latent infection) performed in guinea pigs infected with the auxotrophic mutant remarkably reproduced the response of humans to this test. Together, these findings confirm that infection of guinea pigs with M. tuberculosis strain 18b results in an infectious process that can be used as an interesting alternative model of latent or dormant tuberculosis infection in this animal specie.
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影响因子：
120.7
作者：
Dye, C;Scheele, S;Raviglione, RC
通讯作者：
Raviglione, RC
影响因子：
--
作者：
Kashino, Suely S.;Ovendale, Pamela;Campos-Neto, Antonio
通讯作者：
Campos-Neto, Antonio
影响因子：
158.5
作者：
SMALL, PM;SHAFER, RW;SCHOOLNIK, GK
通讯作者：
SCHOOLNIK, GK
DOI：
10.1164/ajrccm/137.3.716
发表时间：
1988-03-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
ORME, IM
通讯作者：
ORME, IM
影响因子：
6.7
作者：
Miller, MJS;Thompson, JH;Clark, DA
通讯作者：
Clark, DA