Preferential Secretion of R-Type α-Amylase Molecules in Rice Seed Scutellum at High Temperatures
Preferential Secretion of R-Type α-Amylase Molecules in Rice Seed Scutellum at High Temperatures
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高温下水稻种子盾片中R型α-淀粉酶分子的优先分泌
DOI：
10.1104/pp.82.4.880
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发表时间：
1986
期刊：
影响因子：
7.4
通讯作者：
T. Akazawa
中科院分区：
文献类型：
--
作者：
T. Mitsui;T. Akazawa
通过将[35S]蛋氨酸掺入到免疫沉淀产物中，将从4天大的水稻幼苗中取出的新鲜黄斑菌暴露在更高的温度下（40-42℃），可以显著降低α-淀粉酶的生物合成。然而，α-淀粉酶分子的细胞内转运和细胞外分泌在高温下增强，表明α-淀粉酶的生物合成和分泌在温度依赖性上是可区分的。在较高温度下（≧40℃），主要分泌抗内切-β- n -乙酰氨基葡萄糖苷酶H （endo -β-H）水解消化的络合型α-淀粉酶异构体，而在较低温度下（≦15℃），主要分泌易受内切-β-H攻击的异构体。
Exposure of fresh scutella excised from 4-day-old rice seedlings to higher temperatures, (40-42°C), drastically reduced the biosynthesis of α-amylase as determined by the incorporation of [35S]methionine into the immunoprecipitable product. However, the intracellular transport and extracellular secretion of the enzyme molecules were enhanced at high temperatures, indicating that the biosynthesis and secretion of α-amylase are distinguishable in their temperature dependency. At the higher temperature regime (≧40°C), the complex-type α-amylase isoform, resistant to hydrolytic digestion by endo-β-N-acetylglucosaminidase H (Endo-β-H) was predominantly secreted, whereas at lower temperatures (≦15°C), the isoform susceptible to Endo-β-H attack was the major molecular form secreted.
用人肿瘤 DNA 转染 NIH 3T3 后膜糖蛋白糖基化的变化。
影响因子：
11.2
作者：
Santer,UV;Gilbert,F;Glick,MC
通讯作者：
Glick,MC
辛德比斯病毒糖蛋白中表达的寡甘露糖基糖肽的生长依赖性改变。
影响因子：
2.9
作者：
Hakimi,J;Atkinson,PH
通讯作者：
Atkinson,PH