Growth-dependent alterations in oligomannosyl glycopeptides expressed in Sindbis virus glycoproteins.
Growth-dependent alterations in oligomannosyl glycopeptides expressed in Sindbis virus glycoproteins.
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辛德比斯病毒糖蛋白中表达的寡甘露糖基糖肽的生长依赖性改变。
DOI：
10.1021/bi00565a025
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
Atkinson,PH
中科院分区：
文献类型：
--
作者：
Hakimi,J;Atkinson,PH
材料和方法细胞和病毒。原代鸡胚成纤维细胞取自9-11日龄胚胎(Spafas)。胚胎被无菌取出，断头，剔除内脏，并在磷酸盐缓冲盐水中清洗几次。将组织重新悬浮在Eagle的最低基本培养液(Gibco，F-11)中，并强制两次通过50毫升的注射器。组织悬液在37℃，终浓度为0.25%的胰酶(Difco，MI)中孵育，搅拌25分钟。悬浮液穿过棉布，离心(650g)10分钟。细胞悬浮在添加5%胎牛血清、青霉素和链霉素的Eagle‘s培养液中。将相当于2-3个胚胎的量稀释至75mL，倒入生长面积为700-cm2的玻璃滚筒中。将这些细胞培养成融合体，所有的实验都是在CEF中用0.25%胰酶和0.04%EDTA在Earle‘s盐中添加氨基酸和维生素进行的。CEF的生长特征是通过如前所述(Oyama&Eagle，1956；Muramatsu et al.，1973)在37℃的瓶子中跟踪细胞总蛋白32天来建立的，以确定这些细胞的生长和不生长密度抑制状态。CEF在接种后24小时内开始明显生长。细胞生长被监测为玻璃中蛋白质的镁/厘米表面积的量度
Materials and MethodsCells and Viruses. Primary chick embryo fibroblasts were prepared from 9-11-day-old embryos (Spafas). Embryos were removed aseptically, decapitated, eviscerated, and washed several times in phosphate-buffered saline. The tissue was resuspended in Eagle’s minimal essential media (Gibco, F-ll) and forced twicethrough a 50-mL syringe. The tissue sus-pension was incubated at 37 C in a final concentration of 0.25% trypsin (Difco, MI) and stirred for 25 min. The sus-pension was passed through cheesecloth andcentrifuged (650g) for 10 min. Cells were suspended in Eagle’s media supplemented with 5% fetal calf serum, penicillin, and streptomycin. The equivalent of two-three embryos was diluted to 75 mL and poured into glass roller bottles with 700-cm2 growing surface area (Bellco). These cells were grown to confluency, and all experiments described were performed with CEF subdivided with 0.25% trypsin and 0.04% EDTA in Earle’s salts supplemented with amino acids and vitamins. CEF growth characteristics were established by following total cell protein in stoppered-glass T-15 flasks at 37 C for 32 days as previously described (Oyama & Eagle, 1956; Muramatsu et al., 1973) in orderto define growing and nongrowing den-sity-inhibited states of these cells. CEF begin to grow expo-nentially within 24-h postinoculation. Cell growth was mon-itored as a measure of mg of protein/cm2 surface area in glass
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DOI：
10.1016/s0021-9258(17)38161-9
发表时间：
1978-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
I. Tabas;S. Schlesinger;S. Kornfeld
通讯作者：
I. Tabas;S. Schlesinger;S. Kornfeld
水疱性口炎病毒糖蛋白的体外合成和糖基化。
DOI：
10.1073/pnas.74.4.1516
发表时间：
1977
影响因子：
11.1
作者：
F. Toneguzzo;H. Ghosh
通讯作者：
H. Ghosh
病毒蛋白的聚丙烯酰胺凝胶电泳
DOI：
--
发表时间：
1971
期刊：
影响因子：
--
作者：
J. Maizel
通讯作者：
J. Maizel
影响因子：
5.6
作者：
BURGE B W;STRAUSS J H JR
通讯作者：
STRAUSS J H JR
辛德毕斯病毒糖蛋白：宿主细胞对寡糖的影响
影响因子：
5.4
作者：
K. Keegstra;B. Sefton;D. Burke
通讯作者：
D. Burke