Spectral phenotyping of embryonic development reveals integrative thermodynamic responses.
Spectral phenotyping of embryonic development reveals integrative thermodynamic responses.
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DOI：
10.1186/s12859-021-04152-1
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发表时间：
2021-05-06
影响因子：
3
通讯作者：
Rundle SD
中科院分区：
文献类型：
--
作者：
Tills O;Spicer JI;Ibbini Z;Rundle SD
能量代理特征（ept）是一种高维生物体表型的新方法，它量化了与视频平均像素值波动相关的不同时间频率内的能量水平谱。它们在解决生物学中的表型瓶颈方面提供了巨大的潜力，并且在识别致命终点和测量特定功能性状方面有效，但它们在多大程度上可能有助于对表型的额外理解仍然未知。因此，我们在这里测试ept的生物学意义及其相对于基本热力学原理的反应。我们通过在不同温度（20、25和30°C）下的淡水池塘蜗牛（Radix balthica）的整个胚胎发育来实现这一目标，并将反应与Arrhenius方程（Q10 = 2）的预测进行比较。我们发现EPTs是热敏的，它们的频率响应谱使有效的高维治疗在整个生物体发育过程中聚类。ept与热力学预测的温度特异性偏差是明显的，表明了生理上的缓解，尽管它们的反应与人工测量有明显不同。EPT谱在捕获表型预测生物学结果方面是有效的，并表明EPT本身可能反映能量转换水平。整个生物体生物学是非常复杂的，这有助于发展普遍表型方法的挑战。在这里，我们展示了一种新的整体方法的生物学相关性，这种方法不受生物学重要性的先入为主概念的限制。此外，我们发现，即使是最动态的生活史阶段，ept也是一种有效的测量方法。在线版本包含补充材料，可在10.1186/s12859-021-04152-1获得。
Energy proxy traits (EPTs) are a novel approach to high dimensional organismal phenotyping that quantify the spectrum of energy levels within different temporal frequencies associated with mean pixel value fluctuations from video. They offer significant potential in addressing the phenotyping bottleneck in biology and are effective at identifying lethal endpoints and measuring specific functional traits, but the extent to which they might contribute additional understanding of the phenotype remains unknown. Consequently, here we test the biological significance of EPTs and their responses relative to fundamental thermodynamic principles. We achieve this using the entire embryonic development of Radix balthica, a freshwater pond snail, at different temperatures (20, 25 & 30 °C) and comparing responses against predictions from Arrhenius’ equation (Q10 = 2). We find that EPTs are thermally sensitive and their spectra of frequency response enable effective high-dimensional treatment clustering throughout organismal development. Temperature-specific deviation in EPTs from thermodynamic predictions were evident and indicative of physiological mitigation, although they differed markedly in their responses from manual measures. The EPT spectrum was effective in capturing aspects of the phenotype predictive of biological outcomes, and suggest that EPTs themselves may reflect levels of energy turnover. Whole-organismal biology is incredibly complex, and this contributes to the challenge of developing universal phenotyping approaches. Here, we demonstrate the biological relevance of a new holistic approach to phenotyping that is not constrained by preconceived notions of biological importance. Furthermore, we find that EPTs are an effective approach to measuring even the most dynamic life history stages. The online version contains supplementary material available at 10.1186/s12859-021-04152-1.
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影响因子：
2
作者：
Bang, A;Gronkjær, P;Malte, H
通讯作者：
Malte, H
影响因子：
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作者：
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通讯作者：
Fordyce, Polly M.
影响因子：
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作者：
Tills, Oliver;Spicer, John I.;Rundle, Simon D.
通讯作者：
Rundle, Simon D.
影响因子：
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作者：
Rudin-Bitterli TS;Tills O;Spicer JI;Culverhouse PF;Wielhouwer EM;Richardson MK;Rundle SD
通讯作者：
Rundle SD