A combination of pre- and post-exposure ascorbic acid rescues mice from radiation-induced lethal gastrointestinal damage.
A combination of pre- and post-exposure ascorbic acid rescues mice from radiation-induced lethal gastrointestinal damage.
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DOI：
10.3390/ijms141019618
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发表时间：
2013-09-27
影响因子：
5.6
通讯作者：
Takahashi Y
中科院分区：
文献类型：
--
作者：
Ito Y;Kinoshita M;Yamamoto T;Sato T;Obara T;Saitoh D;Seki S;Takahashi Y
开发针对放射引起的胃肠道损伤的有效疗法非常重要，因为它是目前治疗的主要并发症，并且可用的有效疗法很少。尽管我们最近证明抗坏血酸预处理可以减轻受辐射小鼠的致命胃肠道损伤，但超过一半的小鼠最终死亡，因此表明需要更好的方法。然后我们研究了一种更有效的治疗辐射引起的胃肠道损伤的方法。接受 13 Gy 腹部放射的小鼠在放射前三天口服抗坏血酸（250 mg/kg/天）（治疗前），在放射前 8 小时注射一针吞噬剂（250 mg/kg），或在放射后 7 天服用抗坏血酸（治疗后）。由于严重的胃肠道损伤（没有骨髓损伤），对照组小鼠在 13 Gy 的腹部辐射中没有一只存活。抗坏血酸预处理（20% 存活率）、吞噬（20%）和后处理（0%）对受辐射的小鼠均无效。然而，使用抗坏血酸的联合疗法，包括预处理、吞噬和后处理，使所有小鼠免受致命的腹部辐射，并且伴随着胃肠道损伤的显着改善（100%存活）。在抗坏血酸联合治疗中省略后处理会显着降低小鼠的存活率（20% 存活率），这表明抗坏血酸后处理的重要性。与抗坏血酸的联合治疗可能是治疗辐射引起的胃肠道损伤的有效治疗工具。
The development of an effective therapy for radiation-induced gastrointestinal damage is important, because it is currently a major complication of treatment and there are few effective therapies available. Although we have recently demonstrated that pretreatment with ascorbic acid attenuates lethal gastrointestinal damage in irradiated mice, more than half of mice eventually died, thus indicating that better approach was needed. We then investigated a more effective therapy for radiation-induced gastrointestinal damage. Mice receiving abdominal radiation at 13 Gy were orally administered ascorbic acid (250 mg/kg/day) for three days before radiation (pretreatment), one shot of engulfment (250 mg/kg) at 8 h before radiation, or were administered the agent for seven days after radiation (post-treatment). None of the control mice survived the abdominal radiation at 13 Gy due to severe gastrointestinal damage (without bone marrow damage). Neither pretreatment with ascorbic acid (20% survival), engulfment (20%), nor post-treatment (0%) was effective in irradiated mice. However, combination therapy using ascorbic acid, including pretreatment, engulfment and post-treatment, rescued all of the mice from lethal abdominal radiation, and was accompanied by remarkable improvements in the gastrointestinal damage (100% survival). Omitting post-treatment from the combination therapy with ascorbic acid markedly reduced the mouse survival (20% survival), suggesting the importance of post-treatment with ascorbic acid. Combination therapy with ascorbic acid may be a potent therapeutic tool for radiation-induced gastrointestinal damage.
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DOI：
10.1056/nejmct0708278
发表时间：
2008-07-17
期刊：
The New England journal of medicine
影响因子：
--
作者：
Heard KJ
通讯作者：
Heard KJ
DOI：
10.1186/1748-717x-5-22
发表时间：
2010-03-16
期刊：
Radiation oncology (London, England)
影响因子：
--
作者：
Ong ZY;Gibson RJ;Bowen JM;Stringer AM;Darby JM;Logan RM;Yeoh AS;Keefe DM
通讯作者：
Keefe DM
影响因子：
158.5
作者：
CREAGAN, ET;MOERTEL, CG;FRYTAK, S
通讯作者：
FRYTAK, S
影响因子：
3.6
作者：
Berenyi, E;Szendro, ZS;Sulyok, E
通讯作者：
Sulyok, E
DOI：
10.1073/pnas.75.9.4538
发表时间：
1978-01-01
影响因子：
11.1
作者：
CAMERON, E;PAULING, L
通讯作者：
PAULING, L