Species-specific Regulation and Switching of Transcription between Stage-specific Ribosomal RNA Genes in Plasmodium berghei*
Species-specific Regulation and Switching of Transcription between Stage-specific Ribosomal RNA Genes in Plasmodium berghei*
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伯氏疟原虫阶段特异性核糖体 RNA 基因之间的物种特异性调控和转录转换*
DOI：
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发表时间：
1997
影响因子：
4.8
通讯作者：
C. Janse
中科院分区：
文献类型：
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作者：
A. Waters;R. V. van Spaendonk;J. Ramesar;R. Vervenne;R. Dirks;J. Thompson;C. Janse
疟疾寄生虫（疟原虫属）以阶段特异性方式差异表达结构上不同的rRNA基因组。啮齿类疟疾寄生虫伯氏疟原虫的四个rRNA基因形成两类2个单位，它们在遗传上是不连锁的，被称为A型和S型。通过北方分析和原位杂交，表达的单位被证明在同步寄生虫制剂涵盖的发展途径，从血液阶段的无性周期的开始，成熟的动合子的生产。A型单位转录的直接反应细胞生长在血液阶段的无性寄生虫，但在男性（不活跃）和女性（活跃）配子体发生的差异调节。S型表达不仅限于蚊子阶段，并表现出有限的表达期在一个子集的血液阶段滋养体，显着升高的配子体生产寄生虫。与人类寄生虫恶性疟原虫不同，没有证据表明S型转录物的前体形式在配子体中积累。没有观察到显着的rRNA转录培养，受精的动合子，直到20小时的发展时，S-型转录启动。结果进一步表明，在疟原虫中，不同rRNA单位的表达与发育进程有关，但以种属特异性方式。
Malaria parasites (Plasmodium spp.) differentially express structurally distinct sets of rRNA genes in a stage-specific manner. The four rRNA genes of the rodent malaria parasite, P. berghei, form two classes of 2 units that are genetically unlinked and termed A-type and S-type. Through Northern analysis and in situ hybridization, expression of the units was demonstrated in synchronized parasite preparations covering the developmental pathway from the initiation of the blood-stage asexual cycle to the production of mature ookinetes. A-type units were transcribed in direct response to cell growth in bloodstage asexual parasites yet were differentially regulated during male (inactive) and female (active) gametocytogenesis. S-type expression was not confined solely to the mosquito stages and exhibited a finite period of expression in a subset of bloodstage trophozoites that was significantly elevated in gametocyte-producing parasites. Unlike in the human parasite, P. falciparum, there was no evidence for accumulation of precursor forms of the S-type transcripts in gametocytes. No significant rRNA transcription was observed in cultured, fertilized ookinetes until ∼20 h of development when S-type transcription was initiated. The results further demonstrate that in Plasmodium the expression of the different rRNA units is linked to developmental progression but in a species-specific manner.
影响因子：
56.9
作者：
GUNDERSON, JH;SOGIN, ML;MCCUTCHAN, TF
通讯作者：
MCCUTCHAN, TF
聚合酶 I 转录、终止和加工。
影响因子：
--
作者：
Paule,MR
通讯作者：
Paule,MR