Functional and Genome Sequence-Driven Characterization of tal Effector Gene Repertoires Reveals Novel Variants With Altered Specificities in Closely Related Malian Xanthomonas oryzae pv. oryzae Strains.
Functional and Genome Sequence-Driven Characterization of tal Effector Gene Repertoires Reveals Novel Variants With Altered Specificities in Closely Related Malian Xanthomonas oryzae pv. oryzae Strains.
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DOI：
10.3389/fmicb.2018.01657
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发表时间：
2018
影响因子：
5.2
通讯作者：
Cunnac S
中科院分区：
文献类型：
--
作者：
Doucouré H;Pérez-Quintero AL;Reshetnyak G;Tekete C;Auguy F;Thomas E;Koebnik R;Szurek B;Koita O;Verdier V;Cunnac S
水稻白叶枯病（BLB）是由水稻黄单胞菌致病变种（Xanthomonasalbumpv.）Xoo），其将转录激活因子样效应子（TALE）注射到宿主细胞中以调节靶疾病易感基因的表达。Xoo主要毒力TALE普遍靶向SWEET糖转运蛋白家族的易感基因。OsSWEET基因的TALE不响应等位基因已在水稻种质中鉴定或通过基因组编辑创建，并赋予对BLB的抗性。近年来，BLB已成为马里水稻种植的主要生物限制因素之一。为了告知在这个国家部署替代抗性来源，用最近在马里分离的一组菌株挑战携带对TalF（以前的Tal5）或TalC（先前在西非Xoo中鉴定的两个重要TALE）无反应的OsSWEET 14等位基因的水稻品系，并且发现其对这些菌株保持敏感。TALE库的表征显示，talF和talC特异性分子标记同时存在于所有调查的马里菌株中，表明相应的TALE被马里Xoo广泛部署以冗余地靶向OsSWEET 14基因启动子。与此相一致，大多数马里Xoo诱导OsSWEET14的能力不受该基因的talC或talF无应答等位基因的影响。八个马里Xoo基因组的长读段测序和组装证实了活性TalF和TalC变体的广泛存在，并提供了对TALE库多样性的详细了解。所有测序的菌株共有9个进化相关的tal效应基因。值得注意的是，鉴定了不能诱导OsSWEET14的新的TalF变体。此外，两种不同的TalB变体被证明已经失去了同时诱导两种易感基因的能力，如先前报道的来自菌株MAI1和BAI3的该组的创始成员。然而，两种新的TalB变体都保留了诱导两种易感基因中的一种或另一种的能力。这些结果揭示了分子和功能上的差异，并将是重要的广谱和持久的抗BLB在西非的可持续发展。
Rice bacterial leaf blight (BLB) is caused by Xanthomonas oryzae pv. oryzae (Xoo) which injects Transcription Activator-Like Effectors (TALEs) into the host cell to modulate the expression of target disease susceptibility genes. Xoo major-virulence TALEs universally target susceptibility genes of the SWEET sugar transporter family. TALE-unresponsive alleles of OsSWEET genes have been identified in the rice germplasm or created by genome editing and confer resistance to BLB. In recent years, BLB has become one of the major biotic constraints to rice cultivation in Mali. To inform the deployment of alternative sources of resistance in this country, rice lines carrying alleles of OsSWEET14 unresponsive to either TalF (formerly Tal5) or TalC, two important TALEs previously identified in West African Xoo, were challenged with a panel of strains recently isolated in Mali and were found to remain susceptible to these isolates. The characterization of TALE repertoires revealed that talF and talC specific molecular markers were simultaneously present in all surveyed Malian strains, suggesting that the corresponding TALEs are broadly deployed by Malian Xoo to redundantly target the OsSWEET14 gene promoter. Consistent with this, the capacity of most Malian Xoo to induce OsSWEET14 was unaffected by either talC- or talF-unresponsive alleles of this gene. Long-read sequencing and assembly of eight Malian Xoo genomes confirmed the widespread occurrence of active TalF and TalC variants and provided a detailed insight into the diversity of TALE repertoires. All sequenced strains shared nine evolutionary related tal effector genes. Notably, a new TalF variant that is unable to induce OsSWEET14 was identified. Furthermore, two distinct TalB variants were shown to have lost the ability to simultaneously induce two susceptibility genes as previously reported for the founding members of this group from strains MAI1 and BAI3. Yet, both new TalB variants retained the ability to induce one or the other of the two susceptibility genes. These results reveal molecular and functional differences in tal repertoires and will be important for the sustainable deployment of broad-spectrum and durable resistance to BLB in West Africa.
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影响因子：
13.8
作者：
Blanvillain-Baufumé S;Reschke M;Solé M;Auguy F;Doucoure H;Szurek B;Meynard D;Portefaix M;Cunnac S;Guiderdoni E;Boch J;Koebnik R
通讯作者：
Koebnik R
影响因子：
14.9
作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
Eddy SR
影响因子：
4.6
作者：
Grau J;Reschke M;Erkes A;Streubel J;Morgan RD;Wilson GG;Koebnik R;Boch J
通讯作者：
Boch J
影响因子：
56.9
作者：
Boch, Jens;Scholze, Heidi;Bonas, Ulla
通讯作者：
Bonas, Ulla
影响因子：
7.2
作者：
Bezrutczyk, Margaret;Yang, Jungil;Frommer, Wolf B.
通讯作者：
Frommer, Wolf B.