Pyruvate
Pyruvate
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丙酮酸
DOI：
--
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
T. Shimizu
中科院分区：
文献类型：
--
作者：
T. Shimizu
丙酮酸激酶在活细胞中发挥着重要作用。具体地，丙酮酸激酶（PK）是催化糖酵解过程中的最后一步的酶，并且在物种之间高度保守。该酶将磷酸烯醇丙酮酸（PEP）的磷酸基团转移到ADP，从而产生一个ATP和丙酮酸分子。丙酮酸激酶在组织中有各种基因类似物和同种型：L（肝脏）、R（红细胞）、M1（肌肉、心脏和脑）和M2（早期胎儿组织）。丙酮酸激酶缺乏症（PKD）可导致红细胞过早破坏（溶血性贫血）。PKD是非球形红细胞溶血性贫血最常见的遗传原因。丙酮酸激酶的生理学意义需要测定细胞中丙酮酸激酶活性速率的方法。然而，市场上几乎没有可获得且负担得起的PK筛查方法。本研究试图建立一种价格合理、灵敏度高的丙酮酸激酶活性测定方法。我们将产生重组组织特异性丙酮酸激酶，使用亲和层析纯化它们，并优化方法，使用耦合吸光度测定来检查活性。丙酮酸激酶活性测定的发展可以提供丙酮酸激酶活性的见解，促进药物生物化学的影响，如新生儿的PK筛查，并提供一个廉价的工具，用于研究和教育目的。作者：Nhat Q. Truong，Er Y.张培章。
Pyruvate kinase plays an essential role in living cells. Specifically, pyruvate kinase (PK) is an enzyme that catalyzes the last step in the process of glycolysis and is highly conserved among species. The enzyme transfers the phosphate group from phosphoenolpyruvate (PEP) to ADP, thereby generating one molecule of ATP and pyruvate. Pyruvate kinase has various gene analogs and isoforms in tissues: L (liver), R (red blood cells), M1 (muscle, heart, and brain), and M2 (early fetal tissue). Pyruvate kinase deficiency (PKD) may result in the premature destruction of red blood cells (hemolytic anemia). PKD is the most common inherited cause of non-spherocytic hemolytic anemia. The physiological significance of pyruvate kinase necessitates methods to determine the rate of pyruvate kinase activity in cells. However, few accessible and affordable PK screening methods are available on the market. We attempt to develop an affordable and sensitive pyruvate kinase activity assay in this proposed study. We will generate recombinant tissue-specific pyruvate kinase, purify them using affinity chromatography, and optimize methods to examine activities using a coupled absorbance assay. Pyruvate kinase activity assay development may provide insights into the activity of pyruvate kinase, facilitate implications for medicinal biochemistry such as PK screening for new-born babies, and offer an inexpensive tool for research and educational purposes. Author: Nhat Q. Truong, Er Y. Jang, Pei Zhang.
通过在饮食中添加 3-碳化合物以及减肥后高能量再喂养来抑制体重和脂肪的恢复。
DOI：
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发表时间：
1996
期刊：
International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity
影响因子：
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作者：
Stanko,RT;Arch,JE
通讯作者：
Arch,JE
影响因子：
3.3
作者：
STANKO, RT;ROBERTSON, RJ;GOSS, FL
通讯作者：
GOSS, FL
严格限制饮食并辅以二羟基丙酮和丙酮酸的身体成分、能量利用和氮代谢。
DOI：
10.1093/ajcn/55.4.771
发表时间：
1992
期刊：
The American journal of clinical nutrition
影响因子：
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作者：
Stanko,RT;Tietze,DL;Arch,JE
通讯作者：
Arch,JE