Genome-wide DNA methylation analysis reveals phytoestrogen modification of promoter methylation patterns during embryonic stem cell differentiation.
Genome-wide DNA methylation analysis reveals phytoestrogen modification of promoter methylation patterns during embryonic stem cell differentiation.
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DOI：
10.1371/journal.pone.0019278
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发表时间：
2011-04-29
期刊：
影响因子：
3.7
通讯作者：
Muramatsu M
中科院分区：
文献类型：
--
作者：
Sato N;Yamakawa N;Masuda M;Sudo K;Hatada I;Muramatsu M
发育过程中的环境挑战会影响胎儿表观基因组，但易受表观遗传失调影响的时期尚不清楚。通过使用大豆植物雌激素染料木素，这是已知的改变Avy等位基因在胚胎发生过程中的表观遗传状态，我们已经探索了表观遗传调控的敏感期。植入后，当DNA甲基化积极进行时，可以使用小鼠胚胎干（ES）细胞分化系统进行体外分析。小鼠胚胎干细胞在存在或不存在染料木黄酮的情况下分化，通过基于微阵列的启动子甲基化分析和甲基化敏感内切酶（HpaII/McrBC）依赖性富集程序比较第10天的DNA甲基化模式。在染料木素治疗后，在一组启动子中观察到甲基化水平的中度变化。对Ucp1和Sytl1启动子的详细研究进一步揭示，染料木素不影响发生在第0天至第4天的新生甲基化，但会干扰随后的调控过程，导致两个启动子的甲基化水平降低。染料木素干扰分化胚胎干细胞新生甲基化后的甲基化模式。我们的观察表明，对于一个基因子集，早期胚胎中DNA甲基化后的调控可能对染料木黄酮敏感。
Environmental challenges during development affect the fetal epigenome, but the period(s) vulnerable to epigenetic dysregulation is(are) not clear. By employing a soy phytoestrogen, genistein, that is known to alter the epigenetic states of the Avy allele during embryogenesis, we have explored the sensitive period for epigenetic regulation. The post-implantation period, when de novo DNA methylation actively proceeds, is amenable to in vitro analysis using a mouse embryonic stem (ES) cell differentiation system. Mouse ES cells were differentiated in the presence or absence of genistein, and DNA methylation patterns on day 10 were compared by microarray-based promoter methylation analysis coupled with a methylation-sensitive endonuclease (HpaII/McrBC)-dependent enrichment procedure. Moderate changes in methylation levels were observed in a subset of promoters following genistein treatment. Detailed investigation of the Ucp1 and Sytl1 promoters further revealed that genistein does not affect de novo methylation occurring between day 0 and day 4, but interferes with subsequent regulatory processes and leads to a decrease in methylation level for both promoters. Genistein perturbed the methylation pattern of differentiated ES cells after de novo methylation. Our observations suggest that, for a subset of genes, regulation after de novo DNA methylation in the early embryo may be sensitive to genistein.
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影响因子：
3.9
作者：
Saegusa, Chika;Kanno, Eiko;Fukuda, Mitsunori
通讯作者：
Fukuda, Mitsunori
宫内生长限制后，新生儿的胞嘧啶甲基化失调。
影响因子：
3.7
作者：
Einstein F;Thompson RF;Bhagat TD;Fazzari MJ;Verma A;Barzilai N;Greally JM
通讯作者：
Greally JM
在小鼠表观遗传敏感等位基因上DNA甲基化的动态重编程。
影响因子：
4.5
作者：
Blewitt ME;Vickaryous NK;Paldi A;Koseki H;Whitelaw E
通讯作者：
Whitelaw E
影响因子：
14.9
作者：
BIRD, AP
通讯作者：
BIRD, AP
DOI：
10.1073/pnas.0703739104
发表时间：
2007-08-07
影响因子：
11.1
作者：
Dolinoy, Dana C.;Huang, Dale;Jirtle, Randy L.
通讯作者：
Jirtle, Randy L.