Hyperplastic synoviocytes from rats with streptococcal cell wall-induced arthritis exhibit a transformed phenotype that is thymic-dependent and retinoid inhibitable.
Hyperplastic synoviocytes from rats with streptococcal cell wall-induced arthritis exhibit a transformed phenotype that is thymic-dependent and retinoid inhibitable.
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患有链球菌细胞壁诱导性关节炎的大鼠的增生性滑膜细胞表现出胸腺依赖性和类维生素A可抑制的转化表型。
DOI：
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发表时间：
1988
影响因子：
6
通讯作者：
Ronald L. Wilder
中科院分区：
文献类型：
--
作者：
David E. Yocum;Robert Lafyatis;E. Remmers;H. Schumacher;Ronald L. Wilder
已有研究表明，LEW/N大鼠的链球菌细胞壁诱导的关节炎类似于局部肿瘤，部分由成纤维细胞样滑膜细胞的增殖和侵袭性群体组成。为了进一步探索这一概念，对患病大鼠的滑膜细胞进行了原位和体外表征，以获得各种参数的“转化”。滑膜细胞呈梭形分布于整个滑膜，是滑膜侵袭骨、软骨部位的主要细胞类型。它们对波形蛋白染色强烈，波形蛋白是一种微丝，在未成熟和转化的间充质细胞中显著表达。它们对Ia抗原进行了可变染色，没有显示T细胞表面抗原，也没有单核细胞或粒细胞特有的组织化学染色。电子显微镜证实了它们的成纤维细胞样形态，并提示有较高的代谢活性。在原代培养中，异常滑膜细胞贴壁，生长迅速，无接触抑制。此外，它们生长在独立于锚地的条件下。这些异常生长特性被全反式维甲酸抑制。最后，关节炎滑膜的外植体在裸鼠体内形成了短暂的肿瘤样结节。这些观察结合其他数据，支持病理过程代表胸腺依赖的、非恶性的、局部侵袭性炎性肿瘤的概念。
It has been suggested that streptococcal cell wall-induced arthritis in LEW/N rats resembles a localized neoplasm consisting of, in part, a proliferative and invasive population of fibroblast-like synoviocytes. To further pursue this concept, the synoviocytes from diseased rats were characterized in situ and in vitro for various parameters of "transformation." The spindle-shaped synoviocytes were found throughout the synovium and were the predominant cell type at sites of invasion of bone and cartilage by synovium. They stained intensely for vimentin, a microfilament prominently expressed in immature and transformed mesenchymal cells. They stained variably for Ia antigens and did not exhibit T cell surface antigens nor did they stain with histochemical stains characteristic of monocytes or granulocytes. Electron microscopy confirmed their fibroblastlike morphology and suggested high grade metabolic activity. In primary culture, the abnormal synoviocytes were adherent, grew rapidly and did not contact inhibit. Moreover, they grew under anchorage-independent conditions. These abnormal growth characteristics were inhibited by all-trans retinoic acid. Finally, explants of the arthritic synovium formed short-lived tumorlike nodules in athymic nude mice. These observations, considered in the context of other data, support the concept that the pathologic process represents a thymic-dependent, nonmalignant, locally invasive inflammatory neoplasm.
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合成类视黄醇 4-羟基苯基视黄酰胺对大鼠链球菌细胞壁诱导的关节炎具有剂量依赖性抑制作用。
DOI：
10.1016/0192-0561(85)90054-2
发表时间：
1985
期刊：
International journal of immunopharmacology
影响因子：
--
作者：
Haraoui,B;Wilder,RL;Allen,JB;Sporn,MB;Helfgott,RK;Brinckerhoff,CE
通讯作者：
Brinckerhoff,CE
类维生素A对类风湿性关节炎（一种增殖性和侵袭性非恶性疾病）的影响。
DOI：
10.1002/9780470720943.ch12
发表时间：
1985
期刊：
Ciba Foundation symposium
影响因子：
--
作者：
Brinckerhoff,CE;Sheldon,LA;Benoit,MC;Burgess,DR;Wilder,RL
通讯作者：
Wilder,RL
影响因子：
--
作者：
OSULLIVAN, FX;FASSBENDER, HG;KOOPMAN, WJ
通讯作者：
KOOPMAN, WJ
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影响因子：
--
作者：
Brinckerhoff,CE
通讯作者：
Brinckerhoff,CE