Collagen-based cell migration models in vitro and in vivo.
Collagen-based cell migration models in vitro and in vivo.
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DOI：
10.1016/j.semcdb.2009.08.005
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发表时间：
2009-10
影响因子：
7.3
通讯作者：
Friedl P
中科院分区：
文献类型：
--
作者：
Wolf K;Alexander S;Schacht V;Coussens LM;von Andrian UH;van Rheenen J;Deryugina E;Friedl P
纤维胶原是最丰富的细胞外基质（ECM）成分，其维持大多数间质组织和器官（包括皮肤、肠道和乳房）的结构。三维（3D）胶原蛋白结构的密度和空间排列定义了组织的机械性质，即硬度和孔隙率，其在不同的情况下引导或对抗细胞迁移和定位，如形态发生、再生、免疫反应和癌症进展。为了在体外以高的体内保真度再现间质细胞运动，从提取的胶原蛋白单体重构3D胶原蛋白晶格，导致重新组装具有限定孔隙率和刚度的纤维状网状结构。随着肿瘤侵袭研究的重点，我们在这里评估不同的体外胶原蛋白为基础的细胞侵袭模型，采用胃蛋白酶化或非胃蛋白酶化的胶原蛋白提取物，并比较其结构的结缔组织在体内，包括小鼠真皮和乳腺，鸡绒毛尿囊膜（CAM），和人类真皮。使用共聚焦反射和双光子激发的二次谐波发生（SHG）显微镜，我们在这里表明，根据胶原蛋白的来源，在体外模型产生均匀的纤维状纹理与可变的孔径，而所有的体内支架包括一个范围从低到高密度的纤维状网络和异构的孔径在同一组织内。为了更好地了解不同组织环境中间质细胞运动的机制和限制，必须在体外和体内对基于3D ECM的模型之间的结构和物理特性进行深入比较。
Fibrillar collagen is the most abundant extracellular matrix (ECM) constituent which maintains the structure of most interstitial tissues and organs, including skin, gut, and breast. Density and spatial alignment of the three-dimensional (3D) collagen architecture define mechanical tissue properties, i.e. stiffness and porosity, which guide or oppose cell migration and positioning in different contexts, such as morphogenesis, regeneration, immune response, and cancer progression. To reproduce interstitial cell movement in vitro with high in vivo fidelity, 3D collagen lattices are being reconstituted from extracted collagen monomers, resulting in the reassembly of a fibrillar meshwork of defined porosity and stiffness. With a focus on tumor invasion studies, we here evaluate different in vitro collagen-based cell invasion models, employing either pepsinized or un-pepsinized collagen extracts, and compare their structure to connective tissue in vivo, including mouse dermis and mammary gland, chick chorioallantoic membrane (CAM), and human dermis. Using confocal reflection and two-photon-excited second harmonic generation (SHG) microscopy, we here show that, depending on the collagen source, in vitro models yield homogeneous fibrillar texture with variable pore sizes, whereas all in vivo scaffolds comprise a range from low- to high-density fibrillar networks and heterogeneous pore sizes within the same tissue. Future in-depth comparison of structure and physical properties between 3D ECM-based models in vitro and in vivo are mandatory to better understand the mechanisms and limits of interstitial cell movements in distinct tissue environments.
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DOI：
10.1042/bss0700277
发表时间：
2003-01-01
期刊：
PROTEASES AND THE REGULATION OF BIOLOGICAL PROCESSES
影响因子：
--
作者：
Friedl, P;Wolf, K
通讯作者：
Wolf, K
DOI：
10.1083/jcb.200810041
发表时间：
2009-02-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Doyle AD;Wang FW;Matsumoto K;Yamada KM
通讯作者：
Yamada KM
影响因子：
8
作者：
Carragher, N. O.;Walker, S. M.;Frame, M. C.
通讯作者：
Frame, M. C.
影响因子：
3.4
作者：
Campagnola, PJ;Millard, AC;Mohler, WA
通讯作者：
Mohler, WA
DOI：
10.1007/bf01851841
发表时间：
1980-01-01
期刊：
RESEARCH IN EXPERIMENTAL MEDICINE
影响因子：
--
作者：
ENDRICH, B;ASAISHI, K;MESSMER, K
通讯作者：
MESSMER, K