Target-switch SELEX: Screening with alternating targets to generate aptamers to conserved terminal dipeptides.
Target-switch SELEX: Screening with alternating targets to generate aptamers to conserved terminal dipeptides.
复制标题
DOI：
10.1016/j.xpro.2022.101724
复制
发表时间：
2022-12-16
期刊：
影响因子：
--
通讯作者：
Pawlosky, Annalisa
中科院分区：
文献类型：
--
作者：
Cutts, Zachary William;Hong, Jessica M.;Shao, Shirley;Tran, Alexander;Dimon, Michelle;Berndl, Marc;Wu, Diana;Pawlosky, Annalisa
Systematic evolution of ligands by exponential enrichment (SELEX) encompasses a wide variety of high-throughput screening techniques for producing nucleic acid binders to molecular targets through directed evolution. We describe here the design and selection steps for discovery of DNA aptamers with specificity for the two consecutive N-terminal amino acids (AAs) of a small peptide (8–10 amino acids). This bead-based method may be adapted for applications requiring binders which recognize a specific portion of the desired target. For complete details on the use and execution of this protocol, please refer to. SELEX method for discovering aptamers to N-terminal dipeptide Peptide targets with shared 2-amino acid (AA) N-terminus and 6-AA variable region “Switch” runs between 2 targets over 5 selection rounds (ex. target 1, 2, 1…) Ran SELEX in parallel with various targets (“switch”, single target, 2-target mixture) Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Systematic evolution of ligands by exponential enrichment (SELEX) encompasses a wide variety of high-throughput screening techniques for producing nucleic acid binders to molecular targets through directed evolution. We describe here the design and selection steps for discovery of DNA aptamers with specificity for the two consecutive N-terminal amino acids (AAs) of a small peptide (8–10 amino acids). This bead-based method may be adapted for applications requiring binders which recognize a specific portion of the desired target.
登录
查看更多内容
影响因子：
5.8
作者：
Shieh, Kevin R.;Kratschmer, Christina;Golden, Aaron
通讯作者：
Golden, Aaron
DOI：
10.1073/pnas.1009331107
发表时间：
2010-08-31
影响因子：
11.1
作者：
Cho, Minseon;Xiao, Yi;Soh, H. Tom
通讯作者：
Soh, H. Tom
DOI：
10.1007/978-3-319-05269-4_9
发表时间：
2014
期刊：
Research in computational molecular biology : ... Annual International Conference, RECOMB ... : proceedings. RECOMB (Conference : 2005- )
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1007/10_2019_103
发表时间：
2020-01-01
期刊：
APTAMERS IN BIOTECHNOLOGY
影响因子：
--
作者：
Plach, Maximilian;Schubert, Thomas
通讯作者：
Schubert, Thomas
影响因子：
4.6
作者：
Takahashi M;Wu X;Ho M;Chomchan P;Rossi JJ;Burnett JC;Zhou J
通讯作者：
Zhou J