Viral and immunologic examination of human immunodeficiency virus type 1-infected, persistently seronegative persons.
Viral and immunologic examination of human immunodeficiency virus type 1-infected, persistently seronegative persons.
复制标题
对人类免疫缺陷病毒 1 型感染、持续血清阴性者进行病毒和免疫学检查。
DOI：
10.1086/315024
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
R. Lal
中科院分区：
文献类型：
--
作者：
D. Ellenberger;P. Sullivan;J. Dorn;C. Schable;T. Spira;T. Folks;R. Lal
Persons who were human immunodeficiency virus type 1 (HIV-1)-infected but who remained persistently seronegative (HIPS) on HIV-1 antibody tests were examined through AIDS case surveillance. Six such individuals (HIPS-1 to -4, -7, and -9) were examined to determine whether their persistent seronegativity was attributable to immune dysfunction or infection with atypical HIV. Of the 6, 4 had antibody titers to at least 1 other common pathogen. In vitro stimulation of peripheral blood mononuclear cells from HIPS-4 and HIPS-7 with pokeweed mitogen or phosphorothioate oligodeoxynucleotide (direct B cell mitogen) did not produce HIV-1-specific antibody. Reconstitution experiments with recombinant interleukin (rIL)-4 and rIL-12 also had no impact on antibody production. Virus isolates from HIPS-4 and -9 were R5X4-tropic, whereas HIPS-7 was CCR5-tropic only. Sequence analysis of long terminal repeat, p24, and env gp41 did not reveal any specific mutation, and phylogenetic analysis confirmed that all 6 virus specimens were HIV-1 subtype B. These data suggest that the lack of a detectable antibody response in these patients may be the result of immune dysfunction.
影响因子：
56.9
作者：
Rosenberg, ES;Billingsley, JM;Walker, BD
通讯作者：
Walker, BD
影响因子：
1.9
作者：
BACCHETTI, P;JEWELL, NP
通讯作者：
JEWELL, NP