MiR-323b-5p acts as a novel diagnostic biomarker for critical limb ischemia in type 2 diabetic patients.
MiR-323b-5p acts as a novel diagnostic biomarker for critical limb ischemia in type 2 diabetic patients.
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MiR-323b-5p 可作为 2 型糖尿病患者严重肢体缺血的新型诊断生物标志物。
DOI：
10.1038/s41598-018-33310-4
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发表时间：
2018-10-10
影响因子：
4.6
通讯作者：
Deng AP
中科院分区：
文献类型：
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作者：
Cheng B;Li JY;Li XC;Wang XF;Wang ZJ;Liu J;Deng AP
2型糖尿病（T2DM）是外围动脉疾病（PAD）的主要因素，尤其是在临界肢体缺血的情况下（CLI）对于T2DM患者中CLI的当前诊断方法的有限值，我们比较了有或没有CLI的中国T2DM患者的miRNA表达曲线，以发现独特的miRNA是否可以用作潜在的诊断生物标志物。 MiR-210-3p，HSA-MIR-1264，HSA-MIR-323B-5P）在CLI组中被上调，而其他4个miRNA（HSA-MIR-5579-3P，HSA-MIR-665，HSA，HSA，HSA -MIR-4285，HSA-MIR-500A-3P）我们的验证测试被下调miR-323b-5p+混杂风险因素的曲线（AUC）为0.94（95％CI：0.884-0.994，p <0.001），比mir-323b-5p高总之，我们的结果表明，循环HSA-MIR-323B-5P可能是2型糖尿病患者临界肢体缺血的诊断的有前途的血清生物标志物。
Type 2 diabetes mellitus (T2DM) is a major contributor to peripheral artery disease (PAD), especially in cases that advance to critical limb ischemia (CLI). Accumulating evidence indicates that miRNAs play an important role in the development of PAD and T2DM. Due to the limited value of current diagnostic methods for CLI in T2DM patients, we compared the miRNA expression profiles of Chinese T2DM patients with or without CLI to find out whether distinctive miRNAs could serve as potential diagnostic biomarkers. We statistically identified 7 miRNAs (hsa-miR-200b-3p, hsa-miR-2115-3p, hsa-miR-431-5p, hsa-miR-486-5p, hsa-miR-210-3p, hsa-miR-1264, hsa-miR-323b-5p) which were up-regulated in the CLI group, whereas other 4 miRNAs (hsa-miR-5579-3p, hsa-miR-665, hsa-miR-4285, hsa-miR-500a-3p) were down-regulated. Our validation test suggested a relatively high diagnostic accuracy of serum hsa-miR-323b-5p levels for the detection of CLI in T2DM patients, with a sensitivity of 62.67% and a specificity of 80.65%. The area under the curve (AUC) for miR-323b-5p + confounding risk factors was 0.94 (95% CI: 0.884–0.994, P < 0.001), which was higher than that for miR-323b-5p. Taken together, our results indicate that circulating hsa-miR-323b-5p could be a promising serum biomarker for the diagnosis of critical limb ischemia in type 2 diabetic patients.
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DOI：
10.3390/ijerph13111103
发表时间：
2016-11-08
影响因子：
--
作者：
Bąk E;Marcisz C;Kadłubowska M;Michalik A;Krawczyk B;Dobrzyń-Matusiak D;Krzemińska S;Fiałkowski T;Glądys E;Drosdzol-Cop A
通讯作者：
Drosdzol-Cop A
影响因子：
20.1
作者：
Eken, Suzanne M.;Jin, Hong;Maegdefessel, Lars
通讯作者：
Maegdefessel, Lars
影响因子：
3.5
作者：
Cheng, Ming;An, Shoukuan;Li, Junquan
通讯作者：
Li, Junquan
影响因子：
158.5
作者：
CRIQUI, MH;LANGER, RD;BROWNER, D
通讯作者：
BROWNER, D
影响因子：
6.5
作者：
Horswell, Stuart D.;Fryer, Lee G. D.;Shoulders, Carol C.
通讯作者：
Shoulders, Carol C.