L-arginine-nitric oxide pathway involvement in the nerve-evoked relaxant responses of the 5-HT precontracted chick isolated upper oesophagus.
L-arginine-nitric oxide pathway involvement in the nerve-evoked relaxant responses of the 5-HT precontracted chick isolated upper oesophagus.
复制标题
L-精氨酸-一氧化氮途径参与 5-HT 预收缩雏鸡离体上食道的神经诱发松弛反应。
DOI：
10.1111/j.1474-8673.1995.tb00299.x
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
W. Bowman
中科院分区：
文献类型：
--
作者：
M. Gwee;P. Gopalakrishnakone;L. Cheah;W. Bowman
1.河豚毒素（TTx）和选择性一氧化氮合酶（NOS）抑制剂L-硝基精氨酸甲酯（L-NAME）对5-羟色胺（5-HT）预收缩鸡离体食管上段对电场刺激的舒张反应的影响（EFS：25- 30 V，5 Hz，10 s，1 ms脉宽，每100 s）;将食管在1g张力下固定在含有1 μ M阿托品的Krebs溶液中。鸡食管的适当组织切片（30 μ M厚）也进行了NADPH-黄递酶组织化学处理。2. TTx（2 μ M）和L-NAME（100-200 μ M）以浓度依赖性方式抑制5-HT预收缩的鸡食管对EFS的舒张反应; L-Arg（0.5 - 1 mM）可逆转L-NAME的抑制作用，而D-Arg（0.5-1 mM）则无此作用。在没有阿托品和肌张力的情况下，EFS产生的鸡食管收缩反应被1 μ M阿托品完全消除，这也阻断了对乙酰胆碱（50 μ M）的收缩反应。3.在光学显微镜下，NADPH-黄递酶组织化学证实了存在的一氧化氮合酶（NOS）含有神经元和神经纤维在鸡食管。4.因此，5-HT预收缩的鸡离体上食管对EFS的舒张反应是通过刺激非肾上腺素能非胆碱能神经介导的。这些可能对应于组织化学鉴定的含NOS的神经元参与，据推测，在一氧化氮的合成和释放作为松弛剂（抑制性）的神经递质，在这禽平滑肌。
1. The effects of tetrodotoxin (TTx) and the selective nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine methyl ester (L-NAME) on relaxant responses of the 5-HT precontracted chick isolated upper oesophagus to electrical field stimulation (EFS: 25-30V, 5 Hz for 10 s, 1 ms pulse width every 100 s) were investigated; the oesophagus was mounted under 1 g tension in Krebs solution containing 1 microM atropine. Appropriate tissue sections (30 microM thickness) of the chick oesophagus were also processed for NADPH-diaphorase histochemistry. 2. TTx (2 microM) and L-NAME (100-200 microM) inhibited the relaxant responses of the 5-HT precontracted chick oesophagus to EFS in a concentration-dependent manner; L-arginine (0.5-1 mM), but not D-arginine (0.5-1 mM), reversed the inhibition by L-NAME. In the absence of atropine and muscle tone, EFS produced contractile responses of the chick oesophagus that were completely abolished by 1 microM atropine, which also blocked the contractile response to acetylcholine (50 microM). 3. Under light microscopy, NADPH-diaphorase histochemistry confirmed the presence of nitric oxide synthase (NOS)-containing neurones and nerve fibres in the chick oesophagus. 4. The relaxant responses of the 5-HT precontracted chick isolated upper oesophagus to EFS are, therefore, mediated via the stimulation of non-adrenergic non-cholinergic nerves. These are likely to correspond to the histochemically identified NOS-containing neurones involved, presumably, in the synthesis and release of nitric oxide as the relaxant (inhibitory) neurotransmitter in this avian smooth muscle.
影响因子：
56.9
作者：
BURNETT, AL;LOWENSTEIN, CJ;SNYDER, SH
通讯作者：
SNYDER, SH
DOI：
10.1073/pnas.88.17.7797
发表时间：
1991-09-01
影响因子：
11.1
作者：
DAWSON, TM;BREDT, DS;SNYDER, SH
通讯作者：
SNYDER, SH