Determination of prostaglandin profiles in lipopolysaccharide-challenged guinea pig spleen.
Determination of prostaglandin profiles in lipopolysaccharide-challenged guinea pig spleen.
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DOI：
10.1002/bmc.2789
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发表时间：
2013-03
影响因子：
1.8
通讯作者：
Feleder, C.
中科院分区：
文献类型：
--
作者：
Yao, X.;Dai, Y.;Johnson, A.;Hass, M. A.;Feleder, C.
我们之前报道过脂多糖（LPS）刺激豚鼠的脾提取物可以抑制脾切除豚鼠的发热反应，这表明脾脏产生了抑制因子。早期的研究结果表明，该因子是一种脂质。为了确定这一因素，我们分析了对照和lps刺激豚鼠脾提取物中分离的脂质组分，重点是鉴定和量化前列腺素，根据目前的知识，前列腺素可能是生物活性因子。前列腺素广泛参与中枢和外周体温调节，因此这些脂质是脾脏表征的目标。采用固相萃取、薄层色谱（TLC）和高效液相色谱-质谱（HPLC-MS/MS）对脾提取物进行分析。四种前列腺素（PGs、6-酮- pgf1 α、PGF2α、PGE2和PGD2）被鉴定并定量。我们的数据显示，与对照组相比，lps处理的豚鼠脾脏中PG水平增加了一倍。本研究中用于表征脾脏PG的方法比以前用于鉴定和量化脾脏和其他生物组织中PG的免疫测定法具有显着优势。这些方法将用于进一步的研究，以明确表征脾源性PG在LPS诱导的发热反应调节中的作用。
We previously reported that splenic extract from lipopolysaccharide (LPS)-challenged guinea pigs inhibits the exaggerated febrile response of splenectomized guinea pigs, suggesting that the spleen generates an inhibitory factor. Earlier results indicate that the factor is a lipid. In an effort to identify this factor, lipid fractions, isolated from splenic extracts of control and LPS-challenged guinea pigs were analyzed with emphasis on identifying and quantifying prostanoids, which according to current knowledge are the likely bioactive factors. Prostaglandins have been extensively implicated in central and peripheral thermoregulation, and thus these lipids were targeted for characterization in the spleen. Analysis was done on the splenic extracts using solid-phase extraction, analytical and preparative thin layer chromatography (TLC) and high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). Four prostaglandins (PGs, 6-keto-PGF1α, PGF2α, PGE2, and PGD2) were identified and quantified. Our data shows that these PG levels are doubled in LPS-treated guinea pig spleen compared with the control group. The methods used in this investigation to characterize PG in the spleen offer significant advantages over immunoassays previously used to identify and quantify PG in the spleen and other biological tissues. These methods will be utilized in further research needed to definitively characterize the role of splenic-derived PG in modulation of the febrile response induced by LPS.
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DOI：
10.1152/ajpregu.00022.2005
发表时间：
2005-09-01
影响因子：
2.8
作者：
Feleder, C;Perlik, V;Blatteis, CM
通讯作者：
Blatteis, CM
影响因子：
2.9
作者：
HENKE, DC;KOUZAN, S;ELING, TE
通讯作者：
ELING, TE
影响因子：
2.2
作者：
MARSHALL, JC;RIBEIRO, MB;SHEINER, PA
通讯作者：
SHEINER, PA
影响因子：
3.2
作者：
Roland, CR;Nakafusa, Y;Flye, MW
通讯作者：
Flye, MW
DOI：
10.1073/pnas.79.19.6093
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
UENO, R;NARUMIYA, S;HAYAISHI, O
通讯作者：
HAYAISHI, O