Glucocorticoids increase CD4CD25 cell percentage and Foxp3 expression in patients with multiple sclerosis.
Glucocorticoids increase CD4CD25 cell percentage and Foxp3 expression in patients with multiple sclerosis.
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DOI：
10.1111/j.1600-0404.2008.01090.x
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发表时间：
2009-04
影响因子：
3.5
通讯作者：
Constantinescu CS
中科院分区：
文献类型：
--
作者：
Braitch M;Harikrishnan S;Robins RA;Nichols C;Fahey AJ;Showe L;Constantinescu CS
确定多发性硬化（MS）患者糖皮质激素治疗后复发与缓解时CD 4 + CD 25 high T细胞（一组调节性T细胞，Treg）的百分比是否不同，以及复发治疗是否改变Treg群体和Foxp 3（一种关键Treg相关分子）的表达。外周血单核细胞（PBMC）从20例MS患者复发期间，之前和开始类固醇治疗（静脉注射甲泼尼龙1克/天，3天）后2天，然后6周后治疗。采用流式细胞术分析CD 4 + CD 25 hi细胞。通过使用ELISA测量细胞因子，并通过使用定量实时PCR测量Foxp 3、CD 3和CD 25表达。CD 4 + CD 25 hi细胞百分比、血浆IL-10和Foxp 3/CD 3比值在甲基强的松龙开始治疗后48 h升高，并在治疗后6周恢复至基线值。结果表明，糖皮质激素增加Treg细胞功能分子和百分比。这可能是类固醇加速MS复发恢复的机制。
To determine whether percentages of CD4+CD25high T cells (a group of regulatory T cells, Treg) differ in patients with multiple sclerosis (MS) in relapse vs remission after glucocorticoid treatment and whether treatment for relapses changes Treg population and the expression of Foxp3, a key Treg-associated molecule. Peripheral blood mononuclear cells (PBMC) were obtained from 20 patients with MS during relapse, just before and 2 days after starting steroid treatment (i.v. methyl-prednisolone 1 g / day for 3 days) and then 6 weeks after treatment. CD4+CD25hi cells were analysed by using flow cytometry. Cytokines were measured by using an ELISA and Foxp3, CD3 and CD25 expression by using quantitative real-time PCR. The percentage of CD4+CD25hi cells, plasma IL-10 and Foxp3 / CD3 ratio increased 48 h after methylprednisolone initiation and returned to baseline values by 6 weeks post-treatment. Results suggest that glucocorticoids increase Treg cell functional molecules and percentages. This may be a mechanism whereby steroids expedite recovery from MS relapses.
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影响因子：
--
作者：
Leussink, VI;Jung, S;Gold, R
通讯作者：
Gold, R
影响因子：
4.8
作者：
Dhawan, Latika;Liu, Bin;Taubman, Mark B.
通讯作者：
Taubman, Mark B.
影响因子：
3.3
作者：
Gayo, A;Mozo, L;Gutiérrez, C
通讯作者：
Gutiérrez, C
影响因子：
56.9
作者：
Pasare, C;Medzhitov, R
通讯作者：
Medzhitov, R
DOI：
10.1177/1352458507083189
发表时间：
2008-04-01
期刊：
MULTIPLE SCLEROSIS
影响因子：
--
作者：
Cohen, J. A.;Calabresi, P. A.;Zhang, H.
通讯作者：
Zhang, H.