Differential glucocorticoid regulation of the pulmonary hydrophobic surfactant proteins SP-B and SP-C.
Differential glucocorticoid regulation of the pulmonary hydrophobic surfactant proteins SP-B and SP-C.
复制标题
肺疏水性表面活性蛋白 SP-B 和 SP-C 的差异糖皮质激素调节。
DOI：
10.1165/ajrcmb/8.2.222
复制
发表时间：
1993
影响因子：
6.4
通讯作者：
Ballard,PL
中科院分区：
文献类型：
--
作者：
Venkatesh,VC;Iannuzzi,DM;Ertsey,R;Ballard,PL
Materials and MethodsMaterials Waymouth's MB 752/1 medium supplemented with penicillin (100 U/ml), streptomycin (100 pg/ml), and fungizone (2.5 pg/rnl) was obtained from the Cell Culture Facilities, University of California, San Francisco and the University of Pennsylvania. Dex, cytidine, uridine, and other biochemicals were obtained from Sigma Chemical Co.(St. Louis, MO).[3H] uridine (48 Ci/mmol) was obtained from ICN (Costa Mesa, CA) or New England Nuclear (Boston, MA).[32P] UTP (3,000 Ci/mmol) and [32P] CTP were obtained from NEN Research Products (DuPont Co., Wilmington, DE). Restriction and modifying enzymes were obtained from Boehringer Mannheim (Indianapolis, IN) or Bethesda Research Laboratories (Gaithersburg, MD). RNase inhibitor and bluescript plasmid were obtained from Stratagene Cloning Systems (La Jolla, CA).Explant Culture Human fetal lung was obtained from second-trimester therapeutic abortions under protocols approved by the Committees on Human Research at the University of California, San Francisco and the Children's Hospital of Philadelphia. The lung parenchyma was minced into l-mm'bits and cultured as explants in serum-free Waymouth'smedium. The explants were maintained at 37 C in 95% air-5% CO2on a rocker platform as previously described (18). The medium was changed daily. Additives were dissolved in ethanol or culture medium and aliquots added to the culture medium; control cultures received the same amount of diluent. Unless otherwise specified, experiments were carried out on day 4 of culture, when the tissue was established in culture and control levels of SP-B were high.
登录
查看更多内容
编码人肺表面活性剂蛋白脂质的两个 SP-C 基因。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Glasser,SW;Korfhagen,TR;Perme,CM;Pilot-Matias,TJ;Kister,SE;Whitsett,JA
通讯作者：
Whitsett,JA
围产期发育期间和激素治疗后兔肺中表面活性剂相关蛋白信使核糖核酸的水平。
影响因子：
4.8
作者：
Ian Connelly;G. Hammond;P. Harding;F. Possmayer
通讯作者：
F. Possmayer
牛肺表面活性剂纯化低分子量疏水蛋白生物物理活性的比较研究。
DOI：
10.1016/0005-2760(88)90081-1
发表时间：
1988
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Yu Shou;Fred Possmayer
通讯作者：
Fred Possmayer
雌激素稳定卵黄蛋白原 mRNA，防止细胞质降解
影响因子：
64.5
作者：
M. Brock;D. Shapiro
通讯作者：
D. Shapiro
糖皮质激素增强肺表面活性蛋白 B 基因转录。
DOI：
10.1152/ajplung.1991.260.2.l37
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
O'Reilly,MA;Clark,JC;Whitsett,JA
通讯作者：
Whitsett,JA