Human Bronchial Epithelial Cell Growth on Homologous Versus Heterologous Tissue Extracellular Matrix.
Human Bronchial Epithelial Cell Growth on Homologous Versus Heterologous Tissue Extracellular Matrix.
复制标题
DOI：
10.1016/j.jss.2021.01.040
复制
发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Badylak SF
中科院分区：
文献类型：
--
作者：
Ravindra A;D'Angelo W;Zhang L;Reing J;Johnson S;Myerburg M;Badylak SF
细胞外基质（ECM）生物支架的来源组织的脱细胞化已有效地用于许多临床应用。然而，由于在手术植入后立即需要功能性气道上皮，脱细胞气管结构一直不成功。ECM可以溶解形成水凝胶，水凝胶已经显示出支持许多不同细胞类型的生长。本研究的目的是比较气道上皮细胞的能力，附着，形成一个融合的单层和分化同源（气管）和异源（膀胱）ECM基板的潜在应用在全气管置换。对猪气管和膀胱进行脱细胞。人支气管上皮细胞（HBEC）培养分化条件下的脱细胞气管ECM和膀胱基质（UBM）的生物支架和水凝胶，并进行了评估，通过组织学和免疫标记的纤毛，杯状细胞的形成，和基底膜沉积的标志物。气管和膀胱组织均成功脱细胞。HBEC在气管和UBM支架上以及由这些生物支架产生的水凝胶上形成汇合层。细胞生长在气管和UBM水凝胶上，而不是生物支架上，显示出阳性乙酰化微管蛋白染色和粘液产生杯状细胞的存在。IV型胶原免疫标记显示这些细胞在水凝胶表面上的基底膜沉积。ECM水凝胶比脱细胞ECM生物支架更好地支持HBEC的生长和分化，并且显示出作为促进成熟呼吸上皮用于气管中的再生医学应用的底物的潜在效用。
Extracellular matrix (ECM) bioscaffolds produced by decellularization of source tissue have been effectively used for numerous clinical applications. However, decellularized tracheal constructs have been unsuccessful due to the immediate requirement of a functional airway epithelium upon surgical implantation. ECM can be solubilized to form hydrogels that have been shown to support growth of many different cell types. The purpose of the present study is to compare the ability of airway epithelial cells to attach, form a confluent monolayer and differentiate on homologous (trachea) and heterologous (urinary bladder) ECM substrates for potential application in full tracheal replacement. Porcine tracheas and urinary bladders were decellularized. Human bronchial epithelial cells (HBEC) were cultured under differentiation conditions on acellular tracheal ECM and urinary bladder matrix (UBM) bioscaffolds and hydrogels, and were assessed by histology and immunolabeling for markers of ciliation, goblet cell formation, and basement membrane deposition. Both trachea and urinary bladder tissues were successfully decellularized. HBEC formed a confluent layer on both trachea and UBM scaffolds and on hydrogels created from these bioscaffolds. Cells grown on tracheal and UBM hydrogels, but not on bioscaffolds, showed positive acetylated tubulin staining and the presence of mucus-producing goblet cells. Collagen IV immunolabeling showed basement membrane deposition by these cells on the surface of the hydrogels. ECM hydrogels supported growth and differentiation of HBEC better than decellularized ECM bioscaffolds, and show potential utility as substrates for promotion of a mature respiratory epithelium for regenerative medicine applications in the trachea.
登录
查看更多内容
影响因子：
5.6
作者：
Mitash, Nilay;Mu, Fangping;Swiatecka-Urban, Agnieszka
通讯作者：
Swiatecka-Urban, Agnieszka
影响因子：
--
作者：
Hodde, JP;Record, RD;Badylak, SF
通讯作者：
Badylak, SF
影响因子：
3.3
作者：
Lange, P.;Greco, K.;Ansari, T.
通讯作者：
Ansari, T.
DOI：
10.1165/rcmb.2009-0348oc
发表时间：
2010-12-01
影响因子：
6.4
作者：
Myerburg, Michael M.;Harvey, Peter R.;Butterworth, Michael B.
通讯作者：
Butterworth, Michael B.
影响因子：
4.6
作者：
Nieponice, Alejandro;Ciotola, Franco F.;Badaloni, Adolfo E.
通讯作者：
Badaloni, Adolfo E.