Fatty acid composition of ruminal bacteria and protozoa, and effect of defaunation on fatty acid profile in the rumen with special reference to conjugated linoleic acid in cattle.
Fatty acid composition of ruminal bacteria and protozoa, and effect of defaunation on fatty acid profile in the rumen with special reference to conjugated linoleic acid in cattle.
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瘤胃细菌和原生动物的脂肪酸组成，以及动物区系丧失对瘤胃脂肪酸谱的影响，特别是牛的共轭亚油酸。
DOI：
10.1111/j.1740-0929.2010.00854.x
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
H. Itabashi
中科院分区：
文献类型：
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作者：
H. Sultana;K. Miyazawa;S. Kanda;H. Itabashi
本研究的目的是比较瘤胃细菌 (B) 和原生动物 (P) 细胞的脂肪酸 (FA) 组成，并研究原生动物对牛瘤胃 FA 谱的影响。使用三头牛制备瘤胃B和P细胞。使用四头有动物区系的牛和三头丧失动物区系的牛（同父异母的兄弟姐妹）来研究原生动物对瘤胃 FA 谱的影响。 B细胞中C16:0和C18:0占总脂肪酸的比例分别为20.7%和37.4%，而P细胞中的比例分别为33.4%和11.6%。 B细胞中反式牛油酸(VA)和顺式9、反式11共轭亚油酸(CLA)的比例分别为3.9%和1.0%，P细胞中的比例分别为5.5%和1.6%，P细胞中比例较高。 P 细胞中 C18:1、C18:2 和 C18:3 的比例比 B 细胞高两到三倍。动物区系牛B细胞中不饱和脂肪酸、VA和CLA的比例高于动物区系丧失的牛。动物群瘤胃液中的VA和CLA也比丧失动物群高1.6至2.5倍。这种趋势与瘤胃液的无细胞部分相似。这些结果表明原生动物对瘤胃中 VA 和 CLA 的产生有很大贡献。
Objectives of this study were to compare fatty acid (FA) composition of ruminal bacterial (B) and protozoal (P) cells, and to investigate effect of protozoa on FA profile in the rumen of cattle. Three cows were used to prepare ruminal B and P cells. Four faunated and three defaunated cattle (half-siblings) were used to study effect of protozoa on ruminal FA profile. Proportions of C16:0 and C18:0 in total fatty acids in B cells were 20.7% and 37.4%, whereas those in P cells were 33.4% and 11.6%, respectively. Proportions of trans-vaccenic acid (VA) and cis-9, trans-11 conjugated linoleic acid (CLA) in B cells were 3.9% and 1.0%, and those in P cells were 5.5% and 1.6%, respectively, being higher in P cells. Proportions of C18:1, C18:2 and C18:3 in P cells were two to three times higher than in B cells. Proportions of unsaturated fatty acids, VA and CLA in B cells of faunated cattle were higher than those of defaunated. VA and CLA in the ruminal fluid of faunated were also 1.6 to 2.5 times higher than those of defaunated. This tendency was similar for cell-free fraction of ruminal fluid. These results indicate that protozoa contribute greatly in VA and CLA production in the rumen.
饲喂大豆油和亚麻籽油脂肪酸钙盐对荷斯坦奶牛瘤胃液和乳汁中 c9、t11-CLA 产量的影响
DOI：
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发表时间：
2007
期刊：
Asian Australasian J. Animal Science 1
影响因子：
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作者：
Halima;Sultana;Takeshi;Oshida;Toshihiro;Shintaku;Shuhei;Kanda;Hisao;Itabashi
通讯作者：
Itabashi
DOI：
10.1271/bbb.69.499
发表时间：
2005-01
期刊：
Bioscience, Biotechnology, and Biochemistry
影响因子：
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作者：
Yuhei Ozutsumi;K. Tajima;A. Takenaka;H. Itabashi
通讯作者：
Yuhei Ozutsumi;K. Tajima;A. Takenaka;H. Itabashi