Microbial ecology of the hive and pollination landscape: bacterial associates from floral nectar, the alimentary tract and stored food of honey bees (Apis mellifera).
Microbial ecology of the hive and pollination landscape: bacterial associates from floral nectar, the alimentary tract and stored food of honey bees (Apis mellifera).
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DOI：
10.1371/journal.pone.0083125
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Corby-Harris V
中科院分区：
文献类型：
--
作者：
Anderson KE;Sheehan TH;Mott BM;Maes P;Snyder L;Schwan MR;Walton A;Jones BM;Corby-Harris V
几乎所有的真核生物都是肠道中有益或良性细菌的宿主，无论是垂直遗传还是从环境中获得。虽然细菌对蜜蜂肠道的核心作用越来越明显，但蜂巢和授粉环境对蜜蜂微生物健康的影响在很大程度上尚未被探索。在这里，我们比较细菌的花蜜在直接授粉环境，蜜蜂（意大利蜜蜂）消化道的不同部分，并储存在蜂箱（蜂蜜和包装花粉或“蜂饼”）的食物。我们使用培养和测序来探索所有样品类型中的细菌群落，再加上对蜂饼的培养独立分析。我们比较了我们的结果从消化道的文化依赖和文化独立的分析从以前的研究。用标准培养基培养前肠（作物）、中肠和后肠产生了许多相同或高度相似的16S rDNA序列，这些序列是用16S rDNA克隆文库和16S rDNA扩增子的下一代测序发现的。尽管用相同的培养基进行了广泛的培养，但我们的结果并不支持最近研究假设的核心作物细菌群落。我们培养了各种各样的细菌菌株，从7个系统发育组中的6个被认为是蜜蜂后肠的核心。我们的研究结果表明，许多细菌普遍存在于蜜蜂面包和作物也被发现在花蜜，这表明频繁的水平传播。从蜂饼中，我们发现了各种细菌的种类，包括许多可能的病原体和食品腐败生物，以及潜在的有益细菌，包括Kunkeei乳杆菌，醋杆菌科和许多不同的放线菌群。这些细菌对蜂群健康的贡献可能包括一般卫生，真菌和病原体抑制和蜂饼保存。我们的研究结果对于了解环境媒介和核心微生物群对传粉者健康的贡献，以及确定可能影响细菌检测和传播，菌落食物储存和疾病易感性的因素非常重要。
Nearly all eukaryotes are host to beneficial or benign bacteria in their gut lumen, either vertically inherited, or acquired from the environment. While bacteria core to the honey bee gut are becoming evident, the influence of the hive and pollination environment on honey bee microbial health is largely unexplored. Here we compare bacteria from floral nectar in the immediate pollination environment, different segments of the honey bee (Apis mellifera) alimentary tract, and food stored in the hive (honey and packed pollen or “beebread”). We used cultivation and sequencing to explore bacterial communities in all sample types, coupled with culture-independent analysis of beebread. We compare our results from the alimentary tract with both culture-dependent and culture-independent analyses from previous studies. Culturing the foregut (crop), midgut and hindgut with standard media produced many identical or highly similar 16S rDNA sequences found with 16S rDNA clone libraries and next generation sequencing of 16S rDNA amplicons. Despite extensive culturing with identical media, our results do not support the core crop bacterial community hypothesized by recent studies. We cultured a wide variety of bacterial strains from 6 of 7 phylogenetic groups considered core to the honey bee hindgut. Our results reveal that many bacteria prevalent in beebread and the crop are also found in floral nectar, suggesting frequent horizontal transmission. From beebread we uncovered a variety of bacterial phylotypes, including many possible pathogens and food spoilage organisms, and potentially beneficial bacteria including Lactobacillus kunkeei, Acetobacteraceae and many different groups of Actinobacteria. Contributions of these bacteria to colony health may include general hygiene, fungal and pathogen inhibition and beebread preservation. Our results are important for understanding the contribution to pollinator health of both environmentally vectored and core microbiota, and the identification of factors that may affect bacterial detection and transmission, colony food storage and disease susceptibility.
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影响因子：
3.7
作者：
Cornman RS;Tarpy DR;Chen Y;Jeffreys L;Lopez D;Pettis JS;vanEngelsdorp D;Evans JD
通讯作者：
Evans JD
影响因子：
3.7
作者：
Bottacini F;Milani C;Turroni F;Sánchez B;Foroni E;Duranti S;Serafini F;Viappiani A;Strati F;Ferrarini A;Delledonne M;Henrissat B;Coutinho P;Fitzgerald GF;Margolles A;van Sinderen D;Ventura M
通讯作者：
Ventura M
影响因子：
4.2
作者：
Alvarez-Perez, Sergio;Herrera, Carlos M.;de Vega, Clara
通讯作者：
de Vega, Clara
影响因子：
64.8
作者：
Currie, CR;Scott, JA;Malloch, D
通讯作者：
Malloch, D
影响因子：
2.4
作者：
Chen, Yan Ping;Huang, Zachary Y.
通讯作者：
Huang, Zachary Y.