Pathogen webs in collapsing honey bee colonies.
Pathogen webs in collapsing honey bee colonies.
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DOI：
10.1371/journal.pone.0043562
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Evans JD
中科院分区：
文献类型：
--
作者：
Cornman RS;Tarpy DR;Chen Y;Jeffreys L;Lopez D;Pettis JS;vanEngelsdorp D;Evans JD
最近蜜蜂群体的损失在其严重程度、地理分布以及在某些情况下未能呈现已知疾病的公认特征方面都是不寻常的。驯化的蜜蜂面临着许多害虫和病原体，诱人的假设是，群体崩溃是由于暴露于新的或复活的病原体。在这里，我们探讨了目前已知的蜜蜂病原体的发病率和丰度在殖民地遭受蜂群衰竭失调症（CCD），否则弱殖民地，强殖民地来自美国各地。虽然病原体的身份不同，美国东部和西部之间，有一个更大的发病率和丰富的病原体在CCD殖民地。病原体负荷是高度协变的CCD，但不控制蜂箱，这表明CCD殖民地迅速变得容易受到一组不同的病原体，或合并感染可以协同作用，以产生工人的快速消耗的特点的疾病。我们还测试了来自无CCD养蜂场的工人，以确认病原体负荷之间的显着正相关性可以在个体蜜蜂的水平上发展，而不仅仅是CCD的次级效应。这一观察结果和其他最近的数据突出了病原体相互作用作为蜜蜂疾病的重要组成部分。最后，我们使用深度RNA测序来进一步表征CCD和非CCD蜂箱中的微生物多样性。我们发现了最近描述的西奈湖病毒（LSV）的新菌株，并发现了肠道细菌组成变化的证据，这可能是CCD的生物标志物。结果进行了讨论相对于主机-寄生虫的相互作用和其他环境压力的蜜蜂。
Recent losses in honey bee colonies are unusual in their severity, geographical distribution, and, in some cases, failure to present recognized characteristics of known disease. Domesticated honey bees face numerous pests and pathogens, tempting hypotheses that colony collapses arise from exposure to new or resurgent pathogens. Here we explore the incidence and abundance of currently known honey bee pathogens in colonies suffering from Colony Collapse Disorder (CCD), otherwise weak colonies, and strong colonies from across the United States. Although pathogen identities differed between the eastern and western United States, there was a greater incidence and abundance of pathogens in CCD colonies. Pathogen loads were highly covariant in CCD but not control hives, suggesting that CCD colonies rapidly become susceptible to a diverse set of pathogens, or that co-infections can act synergistically to produce the rapid depletion of workers that characterizes the disorder. We also tested workers from a CCD-free apiary to confirm that significant positive correlations among pathogen loads can develop at the level of individual bees and not merely as a secondary effect of CCD. This observation and other recent data highlight pathogen interactions as important components of bee disease. Finally, we used deep RNA sequencing to further characterize microbial diversity in CCD and non-CCD hives. We identified novel strains of the recently described Lake Sinai viruses (LSV) and found evidence of a shift in gut bacterial composition that may be a biomarker of CCD. The results are discussed with respect to host-parasite interactions and other environmental stressors of honey bees.
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影响因子：
4.4
作者：
Cornman SR;Schatz MC;Johnston SJ;Chen YP;Pettis J;Hunt G;Bourgeois L;Elsik C;Anderson D;Grozinger CM;Evans JD
通讯作者：
Evans JD
影响因子：
4.4
作者：
Dainat, Benjamin;Evans, Jay D.;Neumann, Peter
通讯作者：
Neumann, Peter
影响因子：
4.9
作者：
Martinson, Vincent G.;Danforth, Bryan N.;Moran, Nancy A.
通讯作者：
Moran, Nancy A.
DOI：
10.1073/pnas.0906970106
发表时间：
2009-09-01
影响因子：
11.1
作者：
Johnson, Reed M.;Evans, Jay D.;Berenbaum, May R.
通讯作者：
Berenbaum, May R.
影响因子：
3.4
作者：
Evans, Jay D.
通讯作者：
Evans, Jay D.