Properties of alkali-solubilized collagen solution crosslinked by N-hydroxysuccinimide activated adipic acid
Properties of alkali-solubilized collagen solution crosslinked by N-hydroxysuccinimide activated adipic acid
复制标题
N-羟基琥珀酰亚胺活化己二酸交联的碱溶性胶原溶液的性能
DOI：
10.1007/s13367-011-0006-6
复制
发表时间：
2011-04
影响因子：
1.3
通讯作者：
Chen Yihui
中科院分区：
文献类型：
--
作者：
Liu Wentao;Zhang Min;Li Guoying;Chen Yihui
研究了 N-羟基琥珀酰亚胺活化己二酸 (NHS-AA) 对碱溶性胶原蛋白溶液性质的影响。通过三硝基苯磺酸 (TNBS) 测定测定，交联胶原蛋白中的残留氨基含量随着 NHS-AA 浓度的增加而降低。差示扫描量热仪（DSC）结果表明，交联胶原蛋白溶液的最大变性温度（Td）比未交联胶原蛋白溶液（36.6℃）高约4.2℃。此外，通过动态频率扫描获得的储能模量（G′）、损耗模量（G”）和复数粘度（η*）的值随着NHS-AA浓度增加到1.5 mM而增加，然后当进一步增加NHS-AA浓度时略有下降。此外，对于与1.5 mM NHS-AA交联的胶原溶液，动态变性温度（Tdd）比Td（40.8℃）低约1.1℃，应用阿伦尼乌斯型时间-温度叠加（TTS）原理得到的活化能为474.4 kJmol−1。
The effect of N-hydroxysuccinimide activated adipic acid (NHS-AA) on the properties of alkali-solubilized collagen solutions was examined. The residual amino group content in crosslinked collagen, determined by trinitrobenzensulfonic acid (TNBS) assay, was decreased with increasing NHS-AA concentration. The results from differential scanning calorimeter (DSC) indicated that the maximum denaturation temperature (Td) of crosslinked collagen solution was about 4.2°C higher than that of un-crosslinked collagen solution (36.6°C). Moreover, the values of storage modulus (G′), loss modulus (G″) and complex viscosity (η*), obtained by means of dynamic frequency sweeps, were increased as NHS-AA concentration added up to 1.5 mM, and then decreased slightly when further increased NHS-AA concentration. Besides, for collagen solution crosslinked with 1.5 mM NHS-AA, dynamic denaturation temperature (Tdd) was about 1.1°C lower thanTd(40.8°C), and the Arrhenius-type time-temperature superposition (TTS) principle was applied to yield the activation energy to be 474.4 kJmol−1.
登录
查看更多内容
影响因子：
14
作者：
Damink, LHHO;Dijkstra, PJ;Feijen, J
通讯作者：
Feijen, J
影响因子：
5.8
作者：
Dupont-Gillain, CC;Jacquemart, I;Rouxhet, PG
通讯作者：
Rouxhet, PG
浓度和温度对胶原溶液流变行为的影响
DOI：
10.1016/j.ijbiomac.2007.12.010
发表时间：
2008-04-01
影响因子：
8.2
作者：
Lai, Guoli;Li, Yang;Li, Guoying
通讯作者：
Li, Guoying
影响因子：
0.9
作者：
Shuai Zhao;M. Zhang;L. Guoying
通讯作者：
Shuai Zhao;M. Zhang;L. Guoying
胶原分散体/聚乙烯醇共混物的流变行为
影响因子：
1.3
作者：
Lai, Guoli;Du, Zongliang;Li, Guoying
通讯作者：
Li, Guoying