SpoIIID-mediated regulation of σK function during Clostridium difficile sporulation.
SpoIIID-mediated regulation of σK function during Clostridium difficile sporulation.
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DOI：
10.1111/mmi.12856
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发表时间：
2015-01
影响因子：
3.6
通讯作者：
Shen A
中科院分区：
文献类型：
--
作者：
Pishdadian K;Fimlaid KA;Shen A
孢子形成细菌病原体艰难梭菌是全球卫生保健相关腹泻的主要原因。虽然C.虽然艰难梭菌孢子的形成是疾病传播所必需的，但控制这种发育过程的调节途径仅得到部分表征。在被充分研究的芽孢形成菌枯草芽孢杆菌中，高度保守的σE、SpoIIID和σK调节蛋白控制母细胞中的基因表达，以确保正确的芽孢形成。确定C过程中SpoIIID和σK的精确要求。艰难梭菌孢子形成时，我们使用异源表达系统和RNA-Seq转录谱分析了spoIIID和sigK突变体。这些分析表明，sigK从SpoIIID非依赖性启动子的表达在很大程度上绕过了SpoIIID产生耐热孢子的需要。我们还观察到σK在翻译时是活性的，这表明SpoIIID主要起激活sigK的作用。然而，SpoIIID在孢子形成过程中起辅助作用，因为它以σ K依赖的方式提高外孢壁形态发生蛋白CdeC的水平。difficile孢子，表明这些蛋白质调节孢子形成的多个阶段。总的来说，这些结果强调了不同的机制控制厚壁菌门的孢子形成。
The spore-forming bacterial pathogen Clostridium difficile is a leading cause of health-care-associated diarrhea worldwide. Although C. difficile spore formation is essential for disease transmission, the regulatory pathways that control this developmental process have only been partially characterized. In the well-studied spore-former Bacillus subtilis, the highly conserved σE, SpoIIID and σK regulatory proteins control gene expression in the mother cell to ensure proper spore formation. To define the precise requirement for SpoIIID and σK during C. difficile sporulation, we analyzed spoIIID and sigK mutants using heterologous expression systems and RNA-Seq transcriptional profiling. These analyses revealed that expression of sigK from a SpoIIID-independent promoter largely bypasses the need for SpoIIID to produce heat-resistant spores. We also observed that σK is active upon translation, suggesting that SpoIIID primarily functions to activate sigK. SpoIIID nevertheless plays auxiliary roles during sporulation, as it enhances levels of the exosporium morphogenetic protein CdeC in a σK-dependent manner.Analyses of purified spores further revealed that SpoIIID and σK control the adherence of the CotB coat protein to C. difficile spores, indicating that these proteins regulate multiple stages of spore formation. Collectively, these results highlight that diverse mechanisms control spore formation in the Firmicutes.
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影响因子：
9.8
作者：
Eichenberger P;Fujita M;Jensen ST;Conlon EM;Rudner DZ;Wang ST;Ferguson C;Haga K;Sato T;Liu JS;Losick R
通讯作者：
Losick R
影响因子：
6.7
作者：
Frirdich E;Biboy J;Adams C;Lee J;Ellermeier J;Gielda LD;Dirita VJ;Girardin SE;Vollmer W;Gaynor EC
通讯作者：
Gaynor EC
影响因子：
4.8
作者：
Fagan, Robert P.;Fairweather, Neil F.
通讯作者：
Fairweather, Neil F.
影响因子：
5.2
作者：
Deshpande, Abhishek;Pasupuleti, Vinay;Donskey, Curtis J.
通讯作者：
Donskey, Curtis J.
影响因子：
2.2
作者：
Escobar-Cortes, Karina;Barra-Carrasco, Jonathan;Paredes-Sabja, Daniel
通讯作者：
Paredes-Sabja, Daniel