5′ Regulatory region of ubiquitin 2 gene from Porteresia coarctata makes efficient promoters for transgene expression in monocots and dicots
5′ Regulatory region of ubiquitin 2 gene from Porteresia coarctata makes efficient promoters for transgene expression in monocots and dicots
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来自 Porteresia coarctata 的泛素 2 基因的 5 调控区为单子叶植物和双子叶植物中的转基因表达提供有效的启动子
DOI：
10.1007/s00299-013-1416-3
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发表时间：
2013
影响因子：
6.2
通讯作者：
Berlin Heidelberg
中科院分区：
文献类型：
--
作者：
A. Philip;bullet Divya;P. Syamaladevi;bullet M Chakravarthi;bullet K Gopinath;bullet N Subramonian;Ó. Springer;Berlin Heidelberg
关键信息泛素5‘调控区驱动转基因在单子叶和双子叶植物中的表达。摘要泛素启动子是植物中结构性转基因表达的候选基因。在本研究中，我们分离并鉴定了一种新的5‘调控序列泛素基因，该基因是一种耐受胁迫的野生牧草。通过在异源植物系统中的功能分析，我们已经证明了分离的调控片段的全长(Port Ubi2.3)或截断序列(PD2)可以驱动GUS在单子叶和/或双子叶植物中的结构性表达。在对Port Ubi2.3的计算机分析中，发现存在一个640bp的核心启动子区域，紧随其后的是两个外显子和两个内含子，这些外显子和内含子有许多假定的顺式作用位点，分布在整个调节区。6种不同缺失结构在水稻、烟草和甘蔗中的转化和表达研究表明，近端内含子在单子叶和双子叶中都能增强核心启动子的活性，而Port Ubi2.3只能在单子叶中强表达。这个调控序列在结构上与已报道的其他泛素启动子非常不同，与其他常用的启动子-玉米Ubi1和花椰菜花叶病毒35S相比，在单子叶植物中表现更好。
Key messagePorteresiaubiquitin 5′ regulatory region drives transgene expression in monocots and dicots.AbstractUbiquitin promoters are promising candidates for constitutive transgene expression in plants. In this study, we isolated and characterized a novel 5′ regulatory sequence of a ubiquitin gene from Porteresia coarctata, a stress-tolerant wild grass species. Through functional analysis in heterologous plant systems, we have demonstrated that full length (Port Ubi2.3) or truncated sequence (PD2) of the isolated regulatory fragment can drive constitutive expression of GUS in monocots and/or dicots. In silico analysis of Port Ubi2.3 has revealed the presence of a 640 bp core promoter region followed by two exons and two introns with numerous putative cis-acting sites scattered throughout the regulatory region. Transformation and expression studies of six different deletion constructs in rice, tobacco and sugarcane revealed that the proximal intron has an enhancing effect on the activity of the core promoter in both monocots and dicots, whereas, Port Ubi2.3 was able to render strong expression only in monocots. This regulatory sequence is quite distinct from the other reported ubiquitin promoters in structure and performs better in monocots compared to other commonly used promoters—maize Ubi1 and Cauliflower Mosaic Virus 35S.
E-box 元件的作用
影响因子：
2.9
作者：
Voytsekh;Mittag
通讯作者：
Mittag
影响因子：
11.6
作者：
Gowik, Udo;Braeutigam, Andrea;Westhoff, Peter
通讯作者：
Westhoff, Peter
影响因子：
11.6
作者：
MCELROY, D;ZHANG, WG;WU, R
通讯作者：
WU, R