Polyamine-producing bacterium Bacillus megaterium N3 reduced Cd accumulation in wheat and increased the expression of DNA repair- and plant hormone- related proteins in wheat roots
Polyamine-producing bacterium Bacillus megaterium N3 reduced Cd accumulation in wheat and increased the expression of DNA repair- and plant hormone- related proteins in wheat roots
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产生多胺的细菌巨大芽孢杆菌 N3 减少了小麦中镉的积累，并增加了小麦根中 DNA 修复和植物激素相关蛋白的表达
DOI：
10.1016/j.envexpbot.2021.104563
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发表时间：
2021-09
影响因子：
5.7
通讯作者：
Chen Zhaojin
中科院分区：
文献类型：
--
作者：
Qin Shanmei;Wu Xuejiao;Han Hui;Pang Fahu;Zhang Jun;Chen Zhaojin
金属固定细菌在降低植物对金属的吸收方面起着重要作用。然而，很少有研究表明，多胺产生菌对小麦镉吸收的抑制作用。本文采用水培试验研究了巨大芽孢杆菌N3对小麦吸收镉的影响及其对小麦根系差异表达蛋白的影响。结果表明，在3 mg L-1Cd胁迫下，与未接种相比，N3菌株降低了小麦根和叶中Cd含量（分别为33.59%和82.83%）。N3菌株显著降低了Cd在小麦根细胞器和可溶性组分中的比例（24.19%~ 31.02%），提高了Cd在细胞壁中的比例（74.91%）。在3 mg L-1Cd胁迫下，N3菌株还使小麦根和叶中脱落酸和多胺含量增加22.64%~ 372.8%。无标记蛋白质组学分析表明，与镉处理相比，N3+镉处理中有127个和85个蛋白质表达上调和下调。显著富集的项目被鉴定为蛋白质-DNA复合物、DNA包装复合物、细胞间转运、过氧化物酶活性和转移酶活性。此外，在镉胁迫下，与未接种的根相比，在菌株N3的存在下，小麦根中的DNA复制，植物激素和铁锌转运蛋白相关蛋白的表达增加。这些结果为多胺产生菌在小麦安全生产中的应用提供了新的思路和理论依据。
Metal-immobilizing bacteria play an important role in reducing the metal uptake of plants. However, little research has characterized the effects of polyamine-producing bacteria on the inhibition of Cd uptake in wheat. Herein, the effects ofBacillus megateriumN3 on wheat Cd uptake and the differentially expressed proteins in wheat roots were studied using hydroponic experiments. The results showed that under 3 mg L-1Cd stress, strain N3 reduced the Cd content in wheat roots (33.59%) and leaves (82.83%) compared with no inoculation. Strain N3 significantly reduced (24.19%-31.02%) the proportion of Cd in the organelles and the soluble fraction and increased (74.91%) the proportion of Cd in the cell walls of wheat roots. Moreover, the contents of abscisic acid and polyamines in the wheat roots and leaves also increased (22.64%-372.8%) in the presence of strain N3 under 3 mg L-1Cd. Label-free proteomics showed that 127 and 85 proteins were up- and downregulated in the N3+Cd treatment compared with the Cd treatment. The significantly enriched items were identified as a protein-DNA complex, DNA packaging complex, intercellular transport, peroxidase activity, and transferase activity. Moreover, the expression of DNA replication, plant hormone, and Fe-Zn transporter related proteins in wheat roots was increased in the presence of strain N3 compared with uninoculated roots under Cd stress. These results provide new insights and a theoretical basis for applying polyamine-producing bacteria to safe wheat production.
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影响因子：
4.3
作者：
H. Tombuloglu;G. Tombuloglu;M. S. Sakcali;A. Turkan;K. Hakeem;H. Alharby;Shah Fahd;W. Abdul
通讯作者：
H. Tombuloglu;G. Tombuloglu;M. S. Sakcali;A. Turkan;K. Hakeem;H. Alharby;Shah Fahd;W. Abdul
影响因子：
6.1
作者：
Zhipeng Wu;K. Mcgrouther;Dongliang Chen;Wei-dong Wu;Hailong Wang
通讯作者：
Zhipeng Wu;K. Mcgrouther;Dongliang Chen;Wei-dong Wu;Hailong Wang
影响因子：
5.1
作者：
Cui, Guibin;Zhao, Yanfeng;Xi, Yajun
通讯作者：
Xi, Yajun
影响因子：
8.8
作者：
Baltas, Hasan;Sirin, Murat;Ozcelik, Ali Erdem
通讯作者：
Ozcelik, Ali Erdem
影响因子：
8.8
作者：
P. Soudek;M. Ursu;Š. Petrová;T. Vanek
通讯作者：
P. Soudek;M. Ursu;Š. Petrová;T. Vanek