Integral membrane proteins and bilayer proteomics.

Integral membrane proteins and bilayer proteomics.
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DOI:
10.1021/ac303064a
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发表时间:
2013-03-05
影响因子:
7.4
通讯作者:
Whitelegge JP
Whitelegge JP
中科院分区:
化学1区
文献类型:
--
作者:
Whitelegge JP

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整合膜蛋白存在于细胞和细胞器周围的双层膜内,在分子穿过它们的运动以及能量和信号的传导中发挥着关键作用。虽然它们的极端两亲性提出了技术挑战,但生物质谱已应用于膜蛋白化学和生物学的各个方面,包括一级,二级,三级和四级结构的分析,以及伴随功能循环和催化的动力学。
Integral membrane proteins reside within the bilayer membranes that surround cells and organelles, playing critical roles in movement of molecules across them and the transduction of energy and signals. While their extreme amphipathicity presents technical challenges, biological mass spectrometry has been applied to all aspects of membrane protein chemistry and biology, including analysis of primary, secondary, tertiary and quaternary structure, as well as the dynamics that accompany functional cycles and catalysis.
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