Versican knockdown reduces interzone area during early stages of chick synovial joint development.
Versican knockdown reduces interzone area during early stages of chick synovial joint development.
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DOI：
10.1002/ar.21542
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发表时间：
2012-03
影响因子：
2
通讯作者：
Capehart, Anthony A.
中科院分区：
文献类型：
--
作者：
Nagchowdhuri, Partha S.;Andrews, Kristen N.;Robart, Savannah;Capehart, Anthony A.
关键词：
关于指定关节位置的因素已经了解了很多，但是关于这些分子指令如何被翻译成功能性关节组织的知之甚少。以前的研究表明，基质硫酸软骨素蛋白聚糖，多功能蛋白聚糖，在鸡和小鼠的胚胎关节雏形中表现出相似的表达模式，表明在关节发育过程中保守的功能。在本研究中，通过特异性抑制其在早期关节间区、产生关节软骨和关节腔的组织中的表达，研究了多功能蛋白聚糖在关节发育中的重要性。采用卵内显微注射腺病毒shRNA构建体到HH 25鸡翅膀中来沉默发育中的无关节中的内源性多功能蛋白聚糖蛋白。结果显示，在HH 36时，响应于多功能蛋白聚糖敲除，整个安装样本中的非软骨形成肘关节间区面积在统计学上显著（12-14%）减少。组织化学和免疫组织化学分析也发现了关键的versican相关分子，包括透明质酸、腱生蛋白、CD 44和连接蛋白的表达减弱。Versican敲低还降低了假定的关节软骨细胞中的胶原II表达，表明软骨形成可能延迟。结果表明，多功能蛋白聚糖功能与其他基质/细胞表面分子，以促进建立或维持早期关节间区结构。
Much has been learned regarding factors that specify joint placement, but less is known regarding how these molecular instructions are translated into functional joint tissues. Previous studies have shown that the matrix chondroitin sulfate proteoglycan, versican, exhibits a similar pattern of expression in the embryonic joint rudiment of chick and mouse suggesting conserved function during joint development. In the present study versican’s importance in developing joints was investigated by specific inhibition of its expression in the early joint interzone, tissue that gives rise to articular cartilages and joint cavity. In ovo microinjection of adenoviral shRNA constructs into the HH25 chick wing was employed to silence endogenous versican protein in developing appendicular joints. Results showed statistically significant (12–14%) reduction of non-chondrogenic elbow joint interzone area in whole mount specimens at HH36 in response to versican knockdown. Attenuated expression of key versican-associated molecules including hyaluronan, tenascin, CD44, and link protein was also noted by histochemical and immunohistochemical analysis. Versican knockdown also lowered collagen II expression in presumptive articular chondrocytes indicating possible delay in chondrogenesis. Results suggest that versican functions interactively with other matrix/cell surface molecules to facilitate establishment or maintenance of early joint interzone structure.
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影响因子：
2.4
作者：
Ito, MM;Kida, MY
通讯作者：
Kida, MY
影响因子：
2.5
作者：
Kern, Christine B.;Norris, Russell A.;Mjaatvedt, Corey H.
通讯作者：
Mjaatvedt, Corey H.
影响因子：
2.7
作者：
LINSENMA.TF;TOOLE, BP;TRELSTAD, RL
通讯作者：
TRELSTAD, RL
DOI：
10.1073/pnas.94.19.10116
发表时间：
1997-09-16
影响因子：
11.1
作者：
Aspberg, A;Miura, R;Yamaguchi, Y
通讯作者：
Yamaguchi, Y
影响因子：
168.9
作者：
Lee, Chang H.;Cook, James L.;Mendelson, Avital;Moioli, Eduardo K.;Yao, Hai;Mao, Jeremy J.
通讯作者：
Mao, Jeremy J.