Carotenoids from mamey (Pouteria sapota) and carrot (Daucus carota) increase the oxidative stress resistance of Caenorhabditis elegans.
Carotenoids from mamey (Pouteria sapota) and carrot (Daucus carota) increase the oxidative stress resistance of Caenorhabditis elegans.
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DOI：
10.1016/j.bbrep.2021.100989
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发表时间：
2021-07
影响因子：
2.7
通讯作者：
Ortega-Regules AE
中科院分区：
文献类型：
--
作者：
González-Peña MA;Lozada-Ramírez JD;Ortega-Regules AE
类胡萝卜素是在水果和蔬菜中发现的天然色素和抗氧化剂，如胡萝卜、番茄、橙子、芒果、黄玉米、南瓜和mamey。在这项研究中，我们评估了mamey（Pouteria sapota）类胡萝卜素的抗氧化潜力，并将其与胡萝卜（Daucus carota）类胡萝卜素进行比较。从马米和胡萝卜中提取类胡萝卜素，并通过体外（ABTS法）和体内试验（秀丽隐杆线虫抗氧化应激）测定其抗氧化能力。杨梅和胡萝卜类胡萝卜素含量分别为4.42 ± 0.12和5.47 ± 0.04mg β-胡萝卜素/100 g。尽管两种产品中类胡萝卜素含量之间存在差异（p < 0.05），但体外抗氧化能力结果显示两种提取物之间无显著差异（p > 0.05）。杨梅和胡萝卜类胡萝卜素提取物可降低C. elegans分别减少20-30%和30- 40%。两种提取物均能提高线虫的抗性和存活率，且效果优于纯β-胡萝卜素，这可能与类胡萝卜素提取物中的混合物成分复杂有关。这些结果表明，mamey是一个很好的类胡萝卜素的替代来源，它可以保护C。优雅mamey类胡萝卜素的保护作用类似于胡萝卜类胡萝卜素的作用。马米类胡萝卜素显示类似的抗氧化活性，胡萝卜类胡萝卜素。马米和胡萝卜类胡萝卜素提高了C.优雅连续暴露于类胡萝卜素增强了后代的抗性。天然类胡萝卜素混合物的抗氧化效果优于纯β-胡萝卜素。
Carotenoids are natural pigments and antioxidants found in fruits and vegetables such as carrot, tomato, orange, mango, yellow corn, pumpkin, and mamey. In this study, we evaluated the antioxidant potential of mamey (Pouteria sapota) carotenoids and compared them to carrot (Daucus carota) carotenoids. The carotenoids were extracted from mamey and carrot, and their antioxidant capacity were determined via in vitro (ABTS method) and in vivo assays (resistance against oxidative stress in Caenorhabditis elegans). The carotenoid contents in mamey and carrot were 4.42 ± 0.12 and 5.47 ± 0.04 mg β-carotene/100 g, respectively. Despite the differences between the carotenoid contents in both products (p < 0.05), the in vitro antioxidant capacity results showed no significant differences between the extracts (p > 0.05). The mamey and carrot carotenoid extracts decreased the oxidative damage in C. elegans by 20–30% and 30–40%, respectively. Both extracts increased the resistance and enhanced the survival of the nematodes, and showed better effects than pure β-carotene, probably owing to the complex mixture in the carotenoid extracts. These results suggest that mamey is a good alternative source of carotenoids and that it protects against oxidative stress in C. elegans. The protective effect of mamey carotenoids was similar to the effect of carrot carotenoids. Mamey carotenoids displayed similar antioxidant activity to carrot carotenoids. Mamey and carrot carotenoids increased the oxidative stress resistance of C. elegans. Continuous exposure to carotenoids enhanced the resistance of the progeny. Natural mixture of carotenoids had better antioxidant effects than pure β-carotene.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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