Evaluation of Cytotoxicity of Silorane and Methacrylate based Dental Composites using Human Gingival Fibroblasts.
Evaluation of Cytotoxicity of Silorane and Methacrylate based Dental Composites using Human Gingival Fibroblasts.
复制标题
使用人牙龈成纤维细胞评估基于硅烷和甲基丙烯酸酯的牙科复合材料的细胞毒性。
DOI：
--
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
K. Bhat
中科院分区：
文献类型：
--
作者：
Prashanthi Madhyastha;Dilip G Naik;R. Kotian;D. Padma;N. Srikant;K. Bhat
目的
牙科修复材料的沥滤物可能会引起局部和全身不良反应。因此，牙科修复材料的生物和毒性特性必须与口腔组织或一般健康相容。因此，对生物相容性牙科修复材料的需求意味着毒性测试的必要性。本研究的目的是通过细胞毒性测定参数（MTT法）测定和比较硅硼烷基复合材料（Filtek P90）与甲基丙烯酸酯（Z100）对人牙龈成纤维细胞（HGF）的活性、增殖率的可能毒性作用。
材料和方法
获得患者的新鲜健康人牙龈组织活检标本。对于HGF，将细胞在Dulbecco改良Eagle培养基中培养并生长至亚融合单层。达到汇合后，用不同剂量的Filtek P90或Z 100处理细胞不同的时间点。MTT法检测HGF细胞的增殖、活力。
结果
细胞毒性试验结果显示，所有组中前24 h的活细胞百分比非常好，在接下来的48 h内略有下降。然而，在任何给定浓度下，所有组中的增殖率从未低于84%。与阳性对照（丝裂霉素C 250 μg/ml）中细胞存活率的显著降低相比，Filtek P90和Z100处理的细胞在24小时和48小时暴露中均显示出细胞增殖的显著降低。）比较Filtek P90和Z100在24小时和48小时内的毒性，结果表明，在24小时和48小时内，两种复合材料在各浓度下的毒性差异均无显著性（p<0.05）。
结论
综上所述，新的基于硅硼烷的修复复合材料显示出与临床上成功的二甲基丙烯酸酯复合材料相当的细胞毒性特征，表明在口腔环境中无毒，因此有助于这些新的修复材料的临床成功。
AIM
The effects of leached substances from the restorative dental materials may induce local and systemic adverse effects. Thus the biological and toxic properties of the restorative dental materials must be compatible with the oral tissues or with general health. Therefore, the need for biocompatible restorative dental material implies the necessity of toxicity testing. It was the purpose of this investigation to determine and compare the possible toxic effect of silorane based composite (Filtek P90) on human gingival fibroblast (HGF) in vitro using cytotoxicity measuring parameters (MTT assay) in comparison with its methacrylate counterpart (Z100) for their viability, proliferation rate.
MATERIALS AND METHODS
Fresh healthy biopsy specimens of human gingival tissue of patients were obtained. For HGF, cells were cultured in Dulbecco's modified Eagle medium and grown to sub confluent monolayers. After attaining confluence, cells were treated with different doses of the Filtek P90 or Z 100 for different time point. HGF cells were observed for their proliferation, viability by MTT assay.
RESULTS
The results of the cytotoxicity assay showed that, the percentage of viable cells was very good in the first 24h and marginally decreased in the next 48h period in all groups. However, the proliferation rate was never below 84% in all the groups, at any given concentration. Filtek P90 and Z100 treated cells exhibited insignificant decrease in the cell proliferation both in 24h and 48h exposure when compared to significant decrease in the cell survival rate in the positive control (Mitomycin C 250 μg/ml).) Comparison of the toxicity between Filtek P90 and Z100 in 24h & 48h separately showed that there was no significant difference (p<0.05) between these two composites in 24h and 48h' time period at all concentrations of the composites.
CONCLUSION
To conclude, the new silorane based restorative composite showed comparable cytotoxic characteristics to clinically successful dimethacrylate composites suggesting the non-toxic nature in the oral environment and hence contributing to clinical success of these new restorative materials.
影响因子：
5
作者：
Eick, JD;Kostoryz, EL;Yourtee, DM
通讯作者：
Yourtee, DM
影响因子：
2.2
作者：
Nociari, MM;Shalev, A;Russo, C
通讯作者：
Russo, C
影响因子：
4.4
作者：
Eick, J. David;Smith, Robert E.;Kostoryz, Elisabet L.
通讯作者：
Kostoryz, Elisabet L.