Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1α in periodontal ligament cells derived from human permanent teeth in vitro.
Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1α in periodontal ligament cells derived from human permanent teeth in vitro.
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DOI：
10.3892/ijmm.2011.869
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发表时间：
2012-04
影响因子：
5.4
通讯作者：
Hasegawa T
中科院分区：
文献类型：
--
作者：
Asakawa T;Chosa N;Yoshimura Y;Asakawa A;Tanaka M;Ishisaki A;Mitome M;Hasegawa T
虽然来自牙周膜（PDL）组织的细胞被报道具有干细胞样活性，并推测在损伤或正畸治疗后的组织愈合和再生中发挥关键作用，但调节其募集和激活的机制仍不清楚。最近，基质细胞衍生因子1α（SDF-1α）已被报道是重要的干细胞归巢和招募到受伤的网站。本研究旨在探讨成纤维细胞生长因子2（FGF-2）对人恒牙牙周膜细胞SDF-1α表达的影响。实时荧光定量PCR检测结果显示，10 ng/ml FGF-2可抑制PDL细胞SDF-1α mRNA的表达。当用FGF受体1抑制剂SU 5402与FGF-2联合处理PDL细胞时，FGF-2降低的SDF-1α表达被抑制。在JNK抑制剂SP 600125存在下，PDL细胞中SDF-1α mRNA不受FGF-2处理的抑制。Western blot分析也表明，SDF-1α的产生被FGF-2抑制，但在FGF-2 + SU 5402处理后恢复。这些结果表明，来自PDL细胞的SDF-1α通过募集干细胞在牙周组织的再生和稳态中起重要作用。
Although cells derived from periodontal ligament (PDL) tissue are reported to have stem cell-like activity and are speculated to play a crucial role for tissue healing and regeneration after injury or orthodontic treatment, mechanisms regulating their recruitment and activation remain unknown. Recently, stromal cell-derived factor 1α (SDF-1α) has been reported to be important for stem cell homing and recruitment to injured sites. The aim of this study was to evaluate whether fibroblast growth factor 2 (FGF-2) affects the expression of SDF-1α in PDL cells derived from human permanent teeth in vitro. Using real-time PCR, the expression of SDF-1α mRNA in PDL cells was inhibited by treatment with 10 ng/ml FGF-2. When PDL cells were treated with SU5402 (an inhibitor of FGF receptor 1) in combination with FGF-2, the FGF-2-reduced expression of SDF-1α was inhibited. In the presence of the JNK inhibitor SP600125, SDF-1α mRNA in PDL cells was not suppressed by the FGF-2 treatment. Western blot analysis also showed that SDF-1α production was suppressed by treatment with FGF-2, but it recovered with treatment by FGF-2 + SU5402. These findings suggest that SDF-1α from PDL cells plays an important role in the regeneration and homeostasis of periodontal tissues via the recruitment of stem cells.
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影响因子：
2.3
作者：
Kucia, M;Ratajczak, J;Ratajczak, MZ
通讯作者：
Ratajczak, MZ
影响因子：
5.2
作者：
Schmidt, Annette;Ladage, Dennis;Bloch, Wilhelm
通讯作者：
Bloch, Wilhelm
影响因子：
15.9
作者：
BEERTSEN, W;VANDENBOS, T
通讯作者：
VANDENBOS, T
影响因子：
3.5
作者：
Hasegawa, T;Yoshimura, Y;Shirakawa, T
通讯作者：
Shirakawa, T
影响因子：
20.1
作者：
Zernecke, A;Schober, A;Weber, C
通讯作者：
Weber, C