Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis.
Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis.
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DOI：
10.1038/srep10372
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发表时间：
2015-05-21
影响因子：
4.6
通讯作者：
Guan le L
中科院分区：
文献类型：
--
作者：
Guan Y;Liang G;Hawken PA;Malecki IA;Cozens G;Vercoe PE;Martin GB;Guan le L
我们测试了营养不良导致的精子质量下降是否与生殖细胞凋亡增加和精子发生中断有关，以及这些影响是否由小RNA介导。每组8只公羊，在65天内体重增加10%或减少10%。喂养不足增加了睾丸组织中凋亡生殖细胞的数量(P <； 0.05)，增加了凋亡相关基因的表达(P <； 0.05)。我们确定了44个miRNA和35个假定的piRNA在喂养良好和喂养不足的男性中差异表达(FDR <； 0.05)。一些与生殖系统发育、细胞凋亡(MiRNAs)以及精子产生和质量(PiRNAs)有关。新型miR-144(miR-98)被发现针对三个凋亡基因(TP53、CASP3、FASL)。在饲料充足的雄性中，miRNAs占总小RNA的比例高于喂养不足的雄性(P <； 0.0 5)，并且与喂养充分和营养不足的雄性中的piRNA的比例相关(r = 0.8，P <； 0.0 5)。总之，营养不良导致的精子质量下降至少部分是由于支持细胞功能的中断和生殖细胞凋亡的增加，而这是由miRNAs和piRNAs表达的变化所介导的。
We tested whether reductions in spermatozoal quality induced by under-nutrition are associated with increased germ cell apoptosis and disrupted spermatogenesis, and whether these effects are mediated by small RNAs. Groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days. Underfeeding increased the number of apoptotic germ cells (P < 0.05) and increased the expression of apoptosis-related genes (P < 0.05) in testicular tissue. We identified 44 miRNAs and 35 putative piRNAs that were differentially expressed in well-fed and underfed males (FDR < 0.05). Some were related to reproductive system development, apoptosis (miRNAs), and sperm production and quality (piRNAs). Novel-miR-144 (miR-98), was found to target three apoptotic genes (TP53, CASP3, FASL). The proportion of miRNAs as a total of small RNAs was greater in well-fed males than in underfed males (P < 0.05) and was correlated (r = 0.8, P < 0.05) with the proportion of piRNAs in well-fed and underfed males. In conclusion, the reductions in spermatozoal quality induced by under-nutrition are caused, at least partly, by disruptions to Sertoli cell function and increased germ cell apoptosis, mediated by changes in the expression of miRNAs and piRNAs.
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影响因子：
7
作者：
Hausser J;Syed AP;Bilen B;Zavolan M
通讯作者：
Zavolan M
影响因子：
2.8
作者：
CARDOSO, FM;GODINHO, HP
通讯作者：
GODINHO, HP
影响因子：
2.2
作者：
Guan, Yongjuan;Malecki, Irek A.;Martin, Graeme B.
通讯作者：
Martin, Graeme B.
影响因子：
4.5
作者：
Bouhallier, Frantz;Allioli, Nathalie;Rouault, Jean-Pierre
通讯作者：
Rouault, Jean-Pierre
影响因子：
4.4
作者：
Blais, Marie-Eve;Brochu, Sylvie;Perreault, Claude
通讯作者：
Perreault, Claude