Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells.
Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells.
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DOI：
10.1371/journal.pone.0030883
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Smits HH
中科院分区：
文献类型：
--
作者：
van der Vlugt LE;Labuda LA;Ozir-Fazalalikhan A;Lievers E;Gloudemans AK;Liu KY;Barr TA;Sparwasser T;Boon L;Ngoa UA;Feugap EN;Adegnika AA;Kremsner PG;Gray D;Yazdanbakhsh M;Smits HH
血吸虫等慢性蠕虫感染与过敏性疾病呈负相关。在这里，使用B细胞IL-10缺陷小鼠，曼氏血吸虫介导的对实验性卵白蛋白诱导的过敏性气道炎症（AAI）的保护作用被证明是特异性依赖于产生IL-10的B细胞。为了研究所涉及的器官，我们将来自OVA感染小鼠的肺、肠系膜淋巴结或脾的B细胞转移到受体OVA致敏小鼠，并显示肺和脾B细胞都降低AAI，但仅脾B细胞以IL-10依赖的方式降低AAI。虽然脾B细胞保护伴随着肺FoxP 3+调节性T细胞水平的升高，但体内FoxP 3 + T细胞消融仅中度恢复AAI，表明调节性B细胞的直接抑制作用具有重要作用。脾边缘区CD 1d + B细胞被证明是负责的脾B细胞亚群，因为它们在体外产生高水平的IL-10并诱导FoxP 3 + T细胞。事实上，从感染小鼠转移CD 1d + MZ-耗尽的脾B细胞恢复了AAI。明显地，我们发现了一个类似的升高人口的CD 1dhi B细胞在血吸虫感染的加蓬儿童外周血相比，未感染的儿童，这些细胞产生的IL-10水平升高。重要的是，产生IL-10的CD 1dhi B细胞的数量在抗-β-受体抗体治疗后减少。这项研究指出，在小鼠和男性中，溶酶体都有能力驱动产生IL-10的调节性CD 1dhi B细胞的发育，此外，这些细胞有助于减少小鼠的实验性过敏性炎症。
Chronic helminth infections, such as schistosomes, are negatively associated with allergic disorders. Here, using B cell IL-10-deficient mice, Schistosoma mansoni-mediated protection against experimental ovalbumin-induced allergic airway inflammation (AAI) was shown to be specifically dependent on IL-10-producing B cells. To study the organs involved, we transferred B cells from lungs, mesenteric lymph nodes or spleen of OVA-infected mice to recipient OVA-sensitized mice, and showed that both lung and splenic B cells reduced AAI, but only splenic B cells in an IL-10-dependent manner. Although splenic B cell protection was accompanied by elevated levels of pulmonary FoxP3+ regulatory T cells, in vivo ablation of FoxP3+ T cells only moderately restored AAI, indicating an important role for the direct suppressory effect of regulatory B cells. Splenic marginal zone CD1d+ B cells proved to be the responsible splenic B cell subset as they produced high levels of IL-10 and induced FoxP3+ T cells in vitro. Indeed, transfer of CD1d+ MZ-depleted splenic B cells from infected mice restored AAI. Markedly, we found a similarly elevated population of CD1dhi B cells in peripheral blood of Schistosoma haematobium-infected Gabonese children compared to uninfected children and these cells produced elevated levels of IL-10. Importantly, the number of IL-10-producing CD1dhi B cells was reduced after anti-schistosome treatment. This study points out that in both mice and men schistosomes have the capacity to drive the development of IL-10-producing regulatory CD1dhi B cells and furthermore, these are instrumental in reducing experimental allergic inflammation in mice.
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DOI：
10.4049/jimmunol.0803052
发表时间：
2009-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Blair PA;Chavez-Rueda KA;Evans JG;Shlomchik MJ;Eddaoudi A;Isenberg DA;Ehrenstein MR;Mauri C
通讯作者：
Mauri C
影响因子：
15.3
作者：
Karlsson, MR;Rugtveit, J;Brandtzaeg, P
通讯作者：
Brandtzaeg, P
影响因子：
4.4
作者：
Mangan, NE;Fallon, RE;Fallon, PG
通讯作者：
Fallon, PG
影响因子：
14.2
作者：
Shreffler, Wayne G.;Wanich, Niya;Sampson, Hugh A.
通讯作者：
Sampson, Hugh A.
影响因子：
4.4
作者：
Ronet, Catherine;Torre, Yazmin Hauyon-La;Launois, Pascal
通讯作者：
Launois, Pascal