The FlbA-regulated predicted transcription factor Fum21 of Aspergillus niger is involved in fumonisin production.
The FlbA-regulated predicted transcription factor Fum21 of Aspergillus niger is involved in fumonisin production.
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DOI：
10.1007/s10482-017-0952-1
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Wösten HAB
中科院分区：
文献类型：
--
作者：
Aerts D;Hauer EE;Ohm RA;Arentshorst M;Teertstra WR;Phippen C;Ram AFJ;Frisvad JC;Wösten HAB
黑曲霉在整个菌落中分泌蛋白质，形成无性孢子（称为分生孢子）的区域除外。编码 G 蛋白信号调节因子的 flbA 失活会导致菌落无法无性繁殖并在整个菌丝体中分泌蛋白质。此外，ΔflbA菌株表现出细胞裂解并且细胞壁更薄。表达分析表明，38个预测的转录因子基因在菌株ΔflbA中差异表达。在这里，下调最多的预测转录因子基因（称为 fum21）被失活。菌株 Δfum21 的生长、分生孢子和蛋白质分泌不受影响。全基因组表达分析显示，与野生型菌株相比，Δfum21 中分别有 63 个和 11 个基因下调和上调。值得注意的是，预计参与次级代谢的 24 个基因在 Δfum21 中下调，其中包括伏马菌素簇 12 个基因中的 10 个。与此同时，缺失菌株中伏马菌素的产生缺失，吡喃黑苷 A 的产生减少了 25%。总而言之，这些结果将 FlbA 介导的孢子形成抑制分泌与霉菌毒素的产生联系起来。本文的在线版本 (doi:10.1007/s10482-017-0952-1) 包含补充材料，可供授权用户使用。
Aspergillus niger secretes proteins throughout the colony except for the zone that forms asexual spores called conidia. Inactivation of flbA that encodes a regulator of G-protein signaling results in colonies that are unable to reproduce asexually and that secrete proteins throughout the mycelium. In addition, the ΔflbA strain shows cell lysis and has thinner cell walls. Expression analysis showed that 38 predicted transcription factor genes are differentially expressed in strain ΔflbA. Here, the most down-regulated predicted transcription factor gene, called fum21, was inactivated. Growth, conidiation, and protein secretion were not affected in strain Δfum21. Whole genome expression analysis revealed that 63 and 11 genes were down- and up-regulated in Δfum21, respectively, when compared to the wild-type strain. Notably, 24 genes predicted to be involved in secondary metabolism were down-regulated in Δfum21, including 10 out of 12 genes of the fumonisin cluster. This was accompanied by absence of fumonisin production in the deletion strain and a 25% reduction in production of pyranonigrin A. Together, these results link FlbA-mediated sporulation-inhibited secretion with mycotoxin production. The online version of this article (doi:10.1007/s10482-017-0952-1) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Grigoriev IV;Nikitin R;Haridas S;Kuo A;Ohm R;Otillar R;Riley R;Salamov A;Zhao X;Korzeniewski F;Smirnova T;Nordberg H;Dubchak I;Shabalov I
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15
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影响因子：
16.5
作者：
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通讯作者：
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影响因子：
4.1
作者：
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通讯作者：
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影响因子：
3
作者：
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通讯作者：
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